Portable and Label-Free Quantitative Loop-Mediated Isothermal Amplification (LF-qLamp) for Reliable COVID-19 Diagnostics in Three Minutes of Reaction Time: Arduino-Based Detection System Assisted by a pH Microelectrode

被引：10

作者：

Moises Alvarez, Mario ^{[1
,2
]}

Bravo-Gonzalez, Sergio ^{[1
,2
]}

Gonzalez-Gonzalez, Everardo ^{[1
,2
]}

Trujillo-de Santiago, Grissel ^{[1
,3
]}

机构：

[1] Tecnol Monterrey, Ctr Biotecnol FEMSA, Monterrey 64849, Mexico

[2] Tecnol Monterrey, Dept Bioingn, Monterrey 64849, Mexico

[3] Tecnol Monterrey, Dept Ingn Mecatron & Elect, Monterrey 64849, Mexico

来源：

BIOSENSORS-BASEL | 2021年 / 11卷 / 10期

关键词：

SARS-CoV-2; COVID-19; qLAMP; amplification; diagnostic; microelectrodes; point-of-care; DNA; LAMP;

D O I：

10.3390/bios11100386

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Loop-mediated isothermal amplification (LAMP) has been recently studied as an alternative method for cost-effective diagnostics in the context of the current COVID-19 pandemic. Recent reports document that LAMP-based diagnostic methods have a comparable sensitivity and specificity to that of RT-qPCR. We report the use of a portable Arduino-based LAMP-based amplification system assisted by pH microelectrodes for the accurate and reliable diagnosis of SARS-CoV-2 during the first 3 min of the amplification reaction. We show that this simple system enables a straightforward discrimination between samples containing or not containing artificial SARS-CoV-2 genetic material in the range of 10 to 10,000 copies per 50 mu L of reaction mix. We also spiked saliva samples with SARS-CoV-2 synthetic material and corroborated that the LAMP reaction can be successfully monitored in real time using microelectrodes in saliva samples as well. These results may have profound implications for the design of real-time and portable quantitative systems for the reliable detection of viral pathogens including SARS-CoV-2.</p>

引用

页数：14