Development of wheat-alien lines with added Leymus racemosus chromosomes and 6VS/6AL translocation chromosomes

被引：0
作者：
Chen, FD [1 ]
Chen, PD [1 ]
Wang, SL [1 ]
机构：
[1] Nanjing Agr Univ, Minist Agr, Key Lab Crop Cytogenet, Nanjing 210095, Peoples R China
来源：
ACTA BOTANICA SINICA | 2001年 / 43卷 / 04期
关键词：
common wheat; Leymus racemosus; Haynaldia villosa; translocation lines; alien addition lines; bi-color FISH; powdery mildew resistance; scab resistance;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
By chromosome C-banding and bi-color fluorescence in situ hybridization (FISH) using digoxigenin-labelled total genomic DNA of Leymus racemosus (Lam.) Tzvel. and biotinylated total genomic DNA of Haynaldia villosa (L.) Schur as probes, three wheat-alien lines with L. racemosus Lr.7 addition and H. villosa 6VS/6AL translocated chromosomes, and eight lines with L. racemosus Lr. 14 addition and H. villosa 6VS/6AL translocated chromosomes were respectively identified from DALr.7 x T6VS/6AL (93G51-4 x P64) and DALr. 14 x T6VS/6AL (94GI5 x P64) F-2 or F-3 hybrids. Fluorescein-isothiocyanate-conjugated avidin and rhodamine-conjugated sheep anti-digoxigenin Fab fragment were used in bi-color FISH detection. The chromosomes of L. racemosus and 6VS fragment of H. villosa were simultaneously detected by their red and green fluorescence. Powdery mildew and scab resistance were also evaluated. The result showed that the obtained plants had high resistance to these two diseases. The potential usage of bi-color FISH in identifying chromatin of L. racemosus and H. villosa was discussed.
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页码：359 / 363
页数：5
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