Thiophilic interaction chromatography of prostate-specific antigen

被引：13
|
作者：
Chadha, KC [1 ]
Kawinski, E [1 ]
Sulkowski, E [1 ]
机构：
[1] Roswell Pk Canc Inst, Dept Mol & Cellular Biol, Buffalo, NY 14263 USA
来源：
JOURNAL OF CHROMATOGRAPHY B | 2001年 / 754卷 / 02期
关键词：
thiophilic interaction chromatography; prostate-specific antigen;
D O I：
10.1016/S0378-4347(00)00622-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Prostate-specific antigen (PSA) protein and complexes of PSA with alpha (1)-antichymotrypsin (PSA-ACT) or alpha (2)-macroglobulin (PSA-A(2)M) prepared in vitro, have strong affinity for different thiophilic gels (T-gel). Free PSA, and these PSA complexes can be isolated due to their affinity for T-gels. The average recovery of PSA from several of the T-gels, based upon ELISA measurements, was 84 to 94%. The data suggest that T-gel affinity can be explored for the purification of free and complexed PSA from various biologic fluids. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：521 / 525
页数：5
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