Thiophilic interaction chromatography of prostate-specific antigen

Prostate-specific antigen (PSA) protein and complexes of PSA with alpha (1)-antichymotrypsin (PSA-ACT) or alpha (2)-macroglobulin (PSA-A(2)M) prepared in vitro, have strong affinity for different thiophilic gels (T-gel). Free PSA, and these PSA complexes can be isolated due to their affinity for T-gels. The average recovery of PSA from several of the T-gels, based upon ELISA measurements, was 84 to 94%. The data suggest that T-gel affinity can be explored for the purification of free and complexed PSA from various biologic fluids. (C) 2001 Elsevier Science B.V. All rights reserved.