Thiophilic interaction chromatography of prostate-specific antigen

被引：13

作者：

Chadha, KC ^{[1
]}

Kawinski, E ^{[1
]}

Sulkowski, E ^{[1
]}

机构：

[1] Roswell Pk Canc Inst, Dept Mol & Cellular Biol, Buffalo, NY 14263 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 754卷 / 02期

关键词：

thiophilic interaction chromatography; prostate-specific antigen;

D O I：

10.1016/S0378-4347(00)00622-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Prostate-specific antigen (PSA) protein and complexes of PSA with alpha (1)-antichymotrypsin (PSA-ACT) or alpha (2)-macroglobulin (PSA-A(2)M) prepared in vitro, have strong affinity for different thiophilic gels (T-gel). Free PSA, and these PSA complexes can be isolated due to their affinity for T-gels. The average recovery of PSA from several of the T-gels, based upon ELISA measurements, was 84 to 94%. The data suggest that T-gel affinity can be explored for the purification of free and complexed PSA from various biologic fluids. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：521 / 525

页数：5

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