Purification and proteomic identification of putative upstream regulators of polo-like kinase-1 from mitotic cell extracts

被引：9
作者：
Ji, Jae-Hoon [1 ]
Hwang, Hyo-In [1 ]
Lee, Hee-Jae [1 ]
Hyun, Sun-Yi [1 ]
Kang, Hoe-Jin [1 ]
Jang, Young-Joo [1 ]
机构：
[1] Dankook Univ, Lab Cell Cycle & Signal Transduct, WCU Dept NanoBioMed Sci, Inst Tissue Regenerat Engn ITREN, Cheonan Si 330714, Chungnam, South Korea
来源：
FEBS LETTERS | 2010年 / 584卷 / 20期
关键词：
Polo-like kinase-1; Polo-like kinase-1 kinase; Phosphorylation; Mitosis; ANAPHASE-PROMOTING COMPLEX; AURORA-A; PROTEIN-KINASE; SACCHAROMYCES-CEREVISIAE; BOX DOMAIN; PLK; PHOSPHORYLATION; MITOSIS; PLX1; PROGRESSION;
D O I：
10.1016/j.febslet.2010.09.025
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polo-like kinase-1 (Plk1) is phosphorylated on Thr210 for activation during mitosis. Here, we investigated the question of which kinase(s) is the specific upstream kinase of mitotic Plk1. Upstream kinases of Plk1 were purified from mitotic cell extracts through column chromatography procedures, and identified by mass spectrometry. Candidates for Plk1 kinase included p21-activated kinase, aurora A, and mammalian Ste20-like kinases. Immunoprecipitates of these proteins from mitotic cell extracts phosphorylated Plk1 on Thr210. Even if the activity of Aurora A was blocked with a specific inhibitor, Plk1 phosphorylation still occurred, suggesting that function of Plk1 could be controlled by these kinases for proper mitotic progression, as well as by Aurora A in very late G2 phase for the beginning of mitosis. Structured abstract: MINT-7996332: PAK1 (uniprotkb:Q13153) physically interacts (MI:0915) with PLK1 (uniprotkb:P53350) by pull down (MI:0096) MINT-7996345: PAK3 (uniprotkb:O75914) physically interacts (MI:0915) with PLK1 (uniprotkb:P53350) by pull down (MI:0096) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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页码：4299 / 4305
页数：7
相关论文
共 35 条
[1]   HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].
CHEN, C ;
OKAYAMA, H .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752
[2]   Cloning and characterization of a member of the MST subfamily of Ste20-like kinases [J].
Creasy, CL ;
Chernoff, J .
GENE, 1995, 167 (1-2) :303-306
[3]   The molecular basis for phosphodependent substrate targeting and regulation of Plks by the Polo-box domain [J].
Elia, AEH ;
Rellos, P ;
Haire, LF ;
Chao, JW ;
Ivins, FJ ;
Hoepker, K ;
Mohammad, D ;
Cantley, LC ;
Smerdon, SJ ;
Yaffe, MB .
CELL, 2003, 115 (01) :83-95
[4]   Ste20-like kinase (SLK), a regulatory kinase for polo-like kinase (Plk) during the G2/M transition in somatic cells [J].
Ellinger-Ziegelbauer, H ;
Karasuyama, H ;
Yamada, E ;
Tsujikawa, K ;
Todokoro, K ;
Nishida, E .
GENES TO CELLS, 2000, 5 (06) :491-498
[5]   Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1 [J].
Fang, GW ;
Yu, HT ;
Kirschner, MW .
MOLECULAR CELL, 1998, 2 (02) :163-171
[6]   Polo-like kinases: a team that plays throughout mitosis [J].
Glover, DM ;
Hagan, IM ;
Tavares, AAM .
GENES & DEVELOPMENT, 1998, 12 (24) :3777-3787
[7]   POLO-LIKE KINASE IS A CELL-CYCLE-REGULATED KINASE ACTIVATED DURING MITOSIS [J].
HAMANAKA, R ;
SMITH, MR ;
OCONNOR, PM ;
MALOID, S ;
MIHALIC, K ;
SPIVAK, JL ;
LONGO, DL ;
FERRIS, DK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (36) :21086-21091
[8]   Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFβTrCP-dependent destruction of the APC inhibitor Emi1 [J].
Hansen, DV ;
Loktev, AV ;
Ban, KH ;
Jackson, PK .
MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (12) :5623-5634
[9]   Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells [J].
Hirota, T ;
Kunitoku, N ;
Sasayama, T ;
Marumoto, T ;
Zhang, DW ;
Nitta, M ;
Hatakeyama, K ;
Saya, H .
CELL, 2003, 114 (05) :585-598
[10]   Mitotic activation of the kinase Aurora-A requires its binding partner Bora [J].
Hutterer, Andrea ;
Berdnik, Daniela ;
Wirtz-Peitz, Frederik ;
Zigman, Mihaela ;
Schleiffer, Alexander ;
Knoblich, Juergen A. .
DEVELOPMENTAL CELL, 2006, 11 (02) :147-157
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