Enrichment of CD56dimKIR+CD57+ highly cytotoxic NK cells in tumour-infiltrated lymph nodes of melanoma patients

被引：117

作者：

Ali, Talib Hassan ^{[1
,2
]}

Pisanti, Simona ^{[3
,4
]}

Ciaglia, Elena ^{[3
,4
]}

Mortarini, Roberta ^{[5
]}

Anichini, Andrea ^{[5
]}

Garofalo, Cinzia ^{[1
]}

Tallerico, Rossana ^{[1
]}

Santinami, Mario ^{[6
]}

Gulletta, Elio ^{[1
]}

Ietto, Caterina ^{[1
]}

Galgani, Mario ^{[7
]}

Matarese, Giuseppe ^{[3
,8
]}

Bifulco, Maurizio ^{[3
,4
]}

Ferrone, Soldano ^{[9
]}

Colucci, Francesco ^{[10
]}

Moretta, Alessandro ^{[11
]}

Karre, Klas ^{[12
]}

Carbone, Ennio ^{[1
,12
]}

机构：

[1] Univ Catanzaro Magna Graecia, Dept Expt & Clin Med, I-88100 Catanzaro, Italy

[2] Univ Thi Qar, Coll Med, Dept Microbiol, Nasseriah 64001, Iraq

[3] Univ Salerno, Dept Med & Surg, I-84081 Baronissi, Italy

[4] Univ Salerno, Dept Pharm, I-84084 Fisciano, Italy

[5] Fdn IRCCS Ist Nazl Tumori, Dept Expt Oncol & Mol Med, Human Tumors Immunobiol Unit, I-20133 Milan, Italy

[6] Fdn IRCCS Ist Nazl Tumori, Dept Surg, Melanoma & Sarcoma Unit, I-20133 Milan, Italy

[7] Univ Naples Federico II, Dipartimento Med Mol & Biotecnol Med, Ist Endocrinol & Oncol Sperimentale, IEOS CNR, I-80131 Naples, Italy

[8] IRCCS Multimed, I-20128 Milan, Italy

[9] Harvard Univ, Sch Med, Dept Surg, Boston, MA 02115 USA

[10] Univ Cambridge, NIHR Cambridge Biomed Res Ctr, Sch Clin, Dept Obstet & Gynaecol, Cambridge CB2 OSW, England

[11] Univ Genoa, Dept Expt Med, Lab Mol Immunol, I-16126 Genoa, Italy

[12] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, S-17177 Stockholm, Sweden

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

基金：

瑞典研究理事会; 欧洲研究理事会;

关键词：

MONOCLONAL-ANTIBODY; IN-VITRO; RESPONSES; FEATURES;

D O I：

10.1038/ncomms6639

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An important checkpoint in the progression of melanoma is the metastasis to lymph nodes. Here, to investigate the role of lymph node NK cells in disease progression, we analyze frequency, phenotype and functions of NK cells from tumour- infiltrated (TILN) and tumourfree ipsilateral lymph nodes (TFLN) of the same patients. We show an expansion of CD56(dim)CD57(dim)CD69+CCR7+KIR+NK cells in TILN. TILN NK cells display robust cytotoxic activity against autologous melanoma cells. In the blood of metastatic melanoma patients, the frequency of NK cells expressing the receptors for CXCL8 receptor is increased compared with healthy subjects, and blood NK cells also express the receptors for CCL2 and IL- 6. These factors are produced in high amount in TILN and in vitro switch the phenotype of blood NK cells from healthy donors to the phenotype associated with TILN. Our data suggest that the microenvironment of TILN generates and/or recruits a particularly effective NK cell subset.

引用

页数：9

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