Aerobic biodegradation of diethyl phthalate by Acinetobacter sp JDC-16 isolated from river sludge

被引:18
作者
Liang Ren-xing [1 ]
Wu Xue-ling [1 ]
Wang Xing-na [1 ]
Dai Qin-yun [1 ]
Wang Yang-yang [1 ]
机构
[1] Cent South Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China
来源
JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY | 2010年 / 17卷 / 05期
基金
中国国家自然科学基金;
关键词
biodegradation; diethyl phthalate; kinetics; Acinetobacter sp; river sludge; BUTYL-PHTHALATE; ACID ESTERS; DEGRADATION; SOIL; SEDIMENT; PHENOL; PAES;
D O I
10.1007/s11771-010-0584-3
中图分类号
TF [冶金工业];
学科分类号
0806 ;
摘要
A gram negative bacterium, named JDC-16, which can grow well on the substrate of phthalic acid esters (PAEs) as the sole source of carbon and energy, was isolated from river sludge Based on the morphology, physiological and biochemical properties and analysis of 16S rRNA gene sequence, it was preliminarily identified belonging to the genus Acinetobacter The result of substrates utilization range indicates that strain JDC-16 can utilize a variety of phthalates except for diisononyl phthalate (DINP) The degradation tests using diethyl phthalate (DEP) as the model compound show that the optimal pH and temperature for DEP degradation by Acinetobacter sp JDC-16 is 8 0 and 35 C respectively Meanwhile, degradation kinetics under various initial concentrations of DEP reveals that substrate depletion curves fit well with the modified Gompertz model with high correlation coefficient (R-2>0 99) Furthermore, the substrate induction test indicates that DEP-induction can apparently shorten the lag phase and enhance the degradation rate This work highlights the potential of this Isolate for bioremediation of phthalates-contaminated environments
引用
收藏
页码:959 / 966
页数:8
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