Enhancement of the catalytic activity of an artificial phosphotriesterase using a molecular imprinting technique

被引：49

作者：

Meng, ZH ^{[1
]}

Yamazaki, T ^{[1
]}

Sode, K ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 13期

关键词：

artificial enzyme; imidazole; molecular imprinting; Paraoxon; phosphotriesterase;

D O I：

10.1023/A:1024152229526

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An artificial phosphotriesterase (PTE) was constructed by co-polymerization of 4(5)-vinylimidazole-Zn2+-methacrylic acid cluster with a divinylbenzene polymer. Compared with the spontaneous hydrolysis, the resulting polymer catalyst caused 105-fold rate acceleration towards the hydrolysis of diethyl p-nitrophenyl phosphate ( Paraoxon). The catalytic activity of the polymer catalyst could be enhanced for 30% using molecular imprinting technique and the molecularly-imprinted catalyst (MIC) showed a turnover rate of 7.4 x 10(-2) s(-1) towards the hydrolysis of Paraoxon. The MIC also hydrolyzed thiophosphates and phosphorothiolate triester pesticides. Construction of an amperometric sensor employing the MIC as catalyst achieved a detection limit of 0.1 mM Paraoxon.

引用

页码：1075 / 1080

页数：6

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