Development of a novel label-free all-fiber optofluidic biosensor based on Fresnel reflection and its applications

被引：11

作者：

Xu, Wenjuan ^{[1
]}

Zhuo, Yuxin ^{[1
]}

Song, Dan ^{[1
]}

Han, Xiangzhi ^{[1
]}

Xu, Jiaxin ^{[1
]}

Long, Feng ^{[1
]}

机构：

[1] Renmin Univ China, Sch Environm & Nat Resources, Beijing 100872, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2021年 / 1181卷

基金：

中国国家自然科学基金;

关键词：

Refractive index sensor; All-fiber optical system; Fresnel reflection; Optofluidics; SARS-Cov-2; SURFACE-PLASMON RESONANCE; SENSOR;

D O I：

10.1016/j.aca.2021.338910

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel, compact, cost-effective, and robust label-free all-fiber optofluidic biosensor (LF-AOB) based on Fresnel reflection mechanism was built through integrating single-multi mode fiber coupler and highly sensitive micro-photodetector. The Fresnel reflection light intensity detected by the LF-AOB greatly depended on the RI change on the end-surface of the fiber probe according to experimental and simulation results. The capability of the LF-AOB for real-time in situ detection in optofluidic system were verified by measuring salt and protein solution, and the lowest limit of detection was 1.0 x 10-6 RIU. Our proposed theory can effectively eliminate the influence of light intensity fluctuation, and one-point calibration method of sensor performance is conducive for rapid and convenient detection of targets. Label-free sensitive detection of SARS-Cov-2 Spike protein receptor-binding domain (S-RBD) and the binding kinetics assay between S-RBD and anti-S-RBD antibody were achieved using the LF-AOB. These contributed to the elegant design of all-fiber optical system with high efficiency, high resolution and sensitivity of micro-photodetector, and enhanced interaction between the light and the samples at the liquid-sensor interface because of the large surface area of the multi-mode fiber probe. The LF-AOB can be extended as a universal sensing platform to measure other factors associated with refractive index because its high sensitivity, low sample consumption (similar to 160 nL), and capability of real-time in situ detection. (C) 2021 Published by Elsevier B.V.

引用

页数：9