Synthesis and continuous catalytic application of alkaline protease nanoflowers-PVA composite hydrogel

被引：31

作者：

Zhang, Haiyang ^{[1
,2
]}

Fei, Xu ^{[1
]}

Tian, Jing ^{[2
]}

Li, Yao ^{[1
]}

Zhi, Hui ^{[2
]}

Wang, Kang ^{[2
]}

Xu, Longquan ^{[1
]}

Wang, Yi ^{[2
]}

机构：

[1] Dalian Polytech Univ, Instrumental Anal Ctr, 1 Qinggongyuan Rd, Dalian 116034, Peoples R China

[2] Dalian Polytech Univ, Sch Biol Engn, 1 Qinggongyuan Rd, Dalian 116034, Peoples R China

来源：

CATALYSIS COMMUNICATIONS | 2018年 / 116卷

基金：

中国国家自然科学基金;

关键词：

Hybrid nanoflowers; PVA-hydrogels; Alkaline protease; Enzymatic activity; INORGANIC HYBRID NANOFLOWERS; INDUSTRIAL BIOCATALYSTS; ENZYME IMMOBILIZATION; STABILITY; STABILIZATION; STRATEGIES;

D O I：

10.1016/j.catcom.2018.07.015

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This paper reports a facile approach for the synthesis of alkaline protease nanoflowers-poly(vinyl alcohol) (PVA) composite hydrogel (NPCH) from alkaline protease-Cu-3(PO4)(2)center dot 3H(2)O nanoflowers and PVA hydrogel through freezing-thawing. During continuous catalytic application, the PVA hydrogel network protected alkaline protease-Cu-3(PO4)(2)center dot 3H(2)O nanoflowers from damage and helped maintain enzymatic activity at high levels. The enzyme that had been immobilized in nanoflowers and NPCH demonstrated 1027% and 605% higher activity than the free alkaline protease derived from Bacillus licheniformis. When used in cyclic catalysis, NPCH exhibited better reusability than nanoflowers and was easily separated from the product.

引用

页码：5 / 9

页数：5

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