Structural analysis of the intact polysaccharide mannan from Saccharomyces cerevisiae yeast using 1H and 13C NMR spectroscopy at 750 MHz

被引:0
作者
Vinogradov, E [1 ]
Petersen, B [1 ]
Bock, K [1 ]
机构
[1] Carlsberg Lab, Dept Chem, DK-2500 Copenhagen, Denmark
关键词
yeast; mannan; MALDI; HPAE;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mannan from Saccharomyces cerevisiae yeast was studied by high field NMR spectroscopy in an attempt to deduce the structure of the polysaccharide and to assess the ratio of different side chains. The results show that all structural information, agreeing with previously published data, can be extracted by analysis of the NMR spectra of the intact and modified mannan. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:177 / 183
页数:7
相关论文
共 17 条
  • [11] REASSESSMENT OF ANTIGENIC DETERMINANT OF SACCHAROMYCES-CEREVISIAE SEROTYPE-IA
    NISHIKAWA, A
    SEKINE, T
    IKEDA, R
    SHINODA, T
    FUKAZAWA, Y
    [J]. MICROBIOLOGY AND IMMUNOLOGY, 1990, 34 (10) : 825 - 840
  • [12] SHIBATA N, 1997, ARCH BIOCHEM BIOPHYS, V336, P49
  • [13] YEAST FLOCCULATION - RECONCILIATION OF PHYSIOLOGICAL AND GENETIC VIEWPOINTS
    STRATFORD, M
    [J]. YEAST, 1992, 8 (01) : 25 - 38
  • [14] CAUSE AND CONTROL OF FLOCCULATION IN YEAST
    STRAVER, MH
    KIJNE, JW
    SMIT, G
    [J]. TRENDS IN BIOTECHNOLOGY, 1993, 11 (06) : 228 - 232
  • [15] SUPER M, 1992, INFECT AGENT DIS, V1, P194
  • [16] TSAI PK, 1984, J BIOL CHEM, V259, P3805
  • [17] STRUCTURE OF A C-TYPE MANNOSE-BINDING PROTEIN COMPLEXED WITH AN OLIGOSACCHARIDE
    WEIS, WI
    DRICKAMER, K
    HENDRICKSON, WA
    [J]. NATURE, 1992, 360 (6400) : 127 - 134