Structural analysis of the intact polysaccharide mannan from Saccharomyces cerevisiae yeast using 1H and 13C NMR spectroscopy at 750 MHz

被引：0
作者：
Vinogradov, E [1 ]
Petersen, B [1 ]
Bock, K [1 ]
机构：
[1] Carlsberg Lab, Dept Chem, DK-2500 Copenhagen, Denmark
来源：
CARBOHYDRATE RESEARCH | 1998年 / 307卷 / 1-2期
关键词：
yeast; mannan; MALDI; HPAE;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mannan from Saccharomyces cerevisiae yeast was studied by high field NMR spectroscopy in an attempt to deduce the structure of the polysaccharide and to assess the ratio of different side chains. The results show that all structural information, agreeing with previously published data, can be extracted by analysis of the NMR spectra of the intact and modified mannan. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：177 / 183
页数：7
相关论文
共 17 条
  • [1] LOCALIZATION OF ALPHA-1-]3-LINKED MANNOSES IN THE N-LINKED OLIGOSACCHARIDES OF SACCHAROMYCES-CEREVISIAE MNN-MUTANTS
    ALVARADO, E
    BALLOU, L
    HERNANDEZ, LM
    BALLOU, CE
    [J]. BIOCHEMISTRY, 1990, 29 (10) : 2471 - 2482
  • [2] Ballou C, 1976, Adv Microb Physiol, V14, P93, DOI 10.1016/S0065-2911(08)60227-1
  • [3] REVISION OF THE OLIGOSACCHARIDE STRUCTURES OF YEAST CARBOXYPEPTIDASE-Y
    BALLOU, L
    HERNANDEZ, LM
    ALVARADO, E
    BALLOU, CE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (09) : 3368 - 3372
  • [4] Fukazawa Y, 1989, Immunol Ser, V47, P37
  • [5] FUKAZAWA Y, 1980, ZENTRALBL BAKTERIO S, V8, P127
  • [6] HERNANDEZ LM, 1989, J BIOL CHEM, V264, P13648
  • [7] HERNANDEZ LM, 1989, J BIOL CHEM, V264, P11848
  • [8] KJAER M, 1994, METHODS ENZYMOL, V239, P288
  • [9] Structure of a cell wall mannan from the pathogenic yeast, Candida catenulata: Assignment of H-1 nuclear magnetic resonance chemical shifts of the inner alpha-1,6-linked mannose residues substituted by a side chain
    Kobayashi, H
    Suzuki, J
    Tanaka, S
    Kiuchi, Y
    Oyamada, H
    Iwadate, N
    Suzuki, H
    Shibata, N
    Suzuki, S
    Okawa, Y
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1997, 341 (01) : 70 - 74
  • [10] ASSIGNMENT OF H-1 AND C-13 NMR CHEMICAL-SHIFTS OF A D-MANNAN COMPOSED OF ALPHA-(1-]2)-LINKAGE AND ALPHA-(1-]6)-LINKAGE OBTAINED FROM CANDIDA-KEFYR IFO-0586 STRAIN
    KOBAYASHI, H
    WATANABE, M
    KOMIDO, M
    MATSUDA, K
    IKEDAHASEBE, T
    SUZUKI, M
    SHIBATA, N
    HISAMICHI, K
    SUZUKI, S
    [J]. CARBOHYDRATE RESEARCH, 1995, 267 (02) : 299 - 306
12