Cross-triggered and cascaded recycling amplification system for electrochemical detection of circulating microRNA in human serum

被引：33

作者：

Dou, Baoting ^{[1
]}

Zhou, Hui ^{[1
]}

Hong, Yajun ^{[1
]}

Zhao, Liming ^{[1
]}

Wang, Po ^{[1
]}

机构：

[1] Jiangsu Normal Univ, Sch Chem & Mat Sci, Xuzhou 221116, Jiangsu, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2021年 / 57卷 / 58期

基金：

中国国家自然科学基金;

关键词：

NUCLEIC-ACIDS; DNA; PRINCIPLES; MNAZYMES; PLATFORM;

D O I：

10.1039/d1cc02060a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A cross-triggered and cascaded recycling amplification system was developed for electrochemical sensing of microRNA 122 based on the DNAzyme/multicomponent nucleic acid enzyme cleavage technique and a dumbbell-shaped probe. The linear range and detection limit were obtained to be 1 fM-100 pM and 0.34 fM, respectively. Compared with some reported studies, the proposed system can achieve the selective detection of endogenous miRNA in liver injury patients and healthy human serums with the advantages of high sensitivity, low cost, and easy manipulation, which are significant for disease diagnosis as well as the fundamental research of molecular biology.

引用

页码：7116 / 7119

页数：4

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