Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran

被引:25
|
作者
Ola, Antonius R. B. [1 ,2 ]
Metboki, Gema [1 ]
Lay, Caterina S. [1 ]
Sugi, Yoseph [1 ]
De Rozari, Philipi [1 ]
Darmakusuma, Dodi [1 ,2 ]
Hakim, Euis Holisotan [3 ]
机构
[1] Nusa Cendana Univ, Fac Sci & Engn, Chem Dept, Kupang 85001, Indonesia
[2] Nusa Cendana Univ 2, Integrated Res Ctr, Lab UPT LRT Biosains, Kupang 85001, Indonesia
[3] Inst Technol Bandung, Dept Chem, Jl Ganesha 10, Bandung 40132, Indonesia
来源
MOLECULES | 2019年 / 24卷 / 22期
关键词
timor; kojic acid; endophytic fungi; catharanthus roseus; annona squamosa; curcuma xanthorisa; sonneratia alba; SECONDARY METABOLITES; AFLATOXIN; FURAN;
D O I
10.3390/molecules24224200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the year. The endophytic fungi Aspergillus flavus had been isolated from medicinal plants such as Catharanthus roseus, Annona squamosa and Curcuma xanthorisa. The endophytic fungi A. flavus from each plant was cultivated on solid rice media and then analyzed for its capability for producing kojic acid. The production of kojic acid was analyzed by HPLC; the highest amount of kojic acid was observed from the endophytic fungi A. flavus, isolated from the stem of Catharanthus roseus, followed by A. flavus from Annona squamosa and Curcuma xanthorisa. Simple VLC fractionation of the extract of A. flavus from C.roseus led to the isolation of around 11.1 g of pure kojic acid. The structure of kojic acid (1) was confirmed by NMR and MS spectroscopic data. A comparison of the NMR data with the literature supported the revision of the natural product flufuran to kojic acid. To the best of our knowledge, this is the first report of a strain of endophytic fungi producing only kojic acid without any other toxic metabolites such as alfatoxins. Therefore, this Aspergillus flavus strain can be applied as a potential producer of kojic acid for industrial use.
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页数:6
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