Therapeutic effects of moxibustion simultaneously targeting Nrf2 and NF-κB in diabetic peripheral neuropathy

被引:38
作者
Li, Jia [1 ]
Hu, Xia [2 ]
Liang, Fengxia [1 ]
Liu, Jianmin [1 ]
Zhou, Huanjiao [1 ]
Liu, Jiaoping [3 ]
Wang, Hua [1 ]
Tang, Hongtu [1 ]
机构
[1] Hubei Univ Chinese Med, Hubei Prov Collaborat Innovat Ctr Prevent Treatme, Wuhan 430061, Hubei, Peoples R China
[2] Hubei Univ Chinese Med, Coll Basic Med, Wuhan 430061, Hubei, Peoples R China
[3] Huazhong Univ Sci & Technol HUST, Cent Hosp Wuhan, Tongji Med Coll, Dept Oncol,Integrated Chinese & Western Med, Wuhan 430010, Hubei, Peoples R China
关键词
Diabetic peripheral neuropathy; Moxibustion; Neuroinflammation; Cytokine; OXIDATIVE STRESS; ACUPUNCTURE; NEUROINFLAMMATION; PERFORMANCE; MANAGEMENT; RISK; KNEE; RATS;
D O I
10.1007/s12010-019-03052-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Moxibustion is the main alternative medicine treatment that has been beneficial to diabetic peripheral neuropathy (DPN), a common complication secondary to diabetic microvascular injury. However, the underlying protective mechanism of moxibustion against neuroinflammation remains unclear. We hypothesized that moxibustion treats DPN by regulating the balance of nuclear factor-2 erythroid-related factor-2 (Nrf2)-nuclear factor-kappa light chain enhancer of B cells (NF-kB). In vivo, diabetes was induced in rats by injecting streptozotocin (STZ; 60 mg/kg; i.p.). Moxibustion was then applied to "Zusanli" (ST 36), "Guanyuan" (BL 26), and "Yishu" (EX-B 3) acupuncture points. Nerve conduction was detected. Serum interleukin (IL)-1 beta, IL-6, and IL-8 levels were determined through enzyme-linked immunosorbent assay. NF-kappa B and Nrf2 proteins were examined through immunoblot analysis. The mRNA of NF-kappa B and Nrf2 was evaluated through RT-PCR. We found that the conduction velocity and amplitude of the action potentials of sciatic nerve conduction were reduced in the DPN model group but were rescued by moxibustion treatment. Moxibustion also improved the effect of DPN on other parameters, including ultrastructural changes, NF-kappa B and Nrf2 expression in the sciatic nerve, and serum IL-1 beta, IL-6, and IL-8 levels. Our data suggested that moxibustion may alleviate neuroinflammation by inhibiting NF-kappa B and by activating Nrf2. Moxibustion may also provide therapeutic effects for patients with DPN by simultaneously targeting Nrf2 and NF-kappa B.
引用
收藏
页码:1167 / 1182
页数:16
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