Isolation and characterization of Burkholderia sp strain CCA53 exhibiting ligninolytic potential

被引：22

作者：

Akita, Hironaga ^{[1
]}

Kimura, Zen-ichiro ^{[2
]}

Yusoff, Mohd Zulkhairi Mohd ^{[1
,3
]}

Nakashima, Nobutaka ^{[4
,5
]}

Hoshino, Tamotsu ^{[1
,4
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Res Inst Sustainable Chem, 3-11-32 Kagamiyama, Hiroshima 7390046, Japan

[2] Kure Coll, Natl Inst Technol, Dept Civil & Environm Engn, 2-2-11 Aga Minami, Kure, Hiroshima 7378506, Japan

[3] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Serdang 43400, Selangor, Malaysia

[4] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Toyohira Ku, 2-17-2-1 Tsukisamu Higashi, Sapporo, Hokkaido 0628517, Japan

[5] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Meguro Ku, 2-12-1-M6-5 Ookayama, Tokyo 1528550, Japan

来源：

SPRINGERPLUS | 2016年 / 5卷

基金：

日本科学技术振兴机构;

关键词：

16S rRNA gene sequencing; Burkholderia; Lignin-associated aromatic monomer; Lignin-degrading bacterium; SEQUENCE-ANALYSIS; CYSTIC-FIBROSIS; BIODEGRADATION; MULTIVORANS; DEGRADATION; BACTERIA;

D O I：

10.1186/s40064-016-2237-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon source. Alkali lignin and at least eight lignin-associated aromatic monomers supported growth of this strain, and the most effective utilization was observed for p-hydroxybenzene monomers. These findings indicate that Burkholderia sp. strain CCA53 has fragmentary activity for lignin degradation.

引用

页数：5

共 24 条

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