SlMYC1 Regulates Type VI Glandular Trichome Formation and Terpene Biosynthesis in Tomato Glandular Cells

被引:150
作者
Xu, Jiesen [1 ]
van Herwijnen, Zeger O. [2 ]
Drager, Dorthe B. [2 ]
Sui, Chun [1 ,3 ]
Haring, Michel A. [1 ]
Schuurink, Robert C. [1 ]
机构
[1] Univ Amsterdam, Swammerdam Inst Life Sci, Dept Plant Physiol, NL-1098 XH Amsterdam, Netherlands
[2] Rijk Zwaan Breeding BV, Burgemeester Crezeelaan 40, NL-2678 ZG De Lier, Netherlands
[3] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China
关键词
BHLH TRANSCRIPTION FACTORS; JASMONATE-RESPONSIVE EXPRESSION; SESQUITERPENE BIOSYNTHESIS; SPECIALIZED METABOLITES; ALKALOID BIOSYNTHESIS; ARABIDOPSIS TRICHOME; ENHANCED CAROTENOIDS; SOLANUM-HABROCHAITES; GENE; LYCOPERSICON;
D O I
10.1105/tpc.18.00571
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tomato (Solanum lycopersicum) glandular trichomes function as biochemical factories that synthesize a diverse array of specialized metabolites. Terpenoids are the most diverse class of plant specialized metabolites, with volatile mono- and sesquiterpenes playing important roles in plant defense. Although the biosynthetic pathways of volatile terpenes in tomato glandular trichomes have been well described, little is known about their regulation. Here, we demonstrate that SlMYC1, a basic helix-loop-helix transcription factor, differentially regulates mono- and sesquiterpene biosynthesis in the type VI glandular trichomes of tomato leaves and stems. SlMYC1 functions as a positive regulator of monoterpene biosynthesis in both leaf and stem trichomes but as a negative regulator of sesquiterpene biosynthesis in stem trichomes. SlMYC1 is also essential for type VI glandular trichome development, as knocking down SlMYC1 led to the production of smaller type VI glandular trichomes at lower densities, and knocking out this gene led to their absence. Our findings reveal a role for SlMYC1 not only in type VI glandular trichome development but also in the regulation of terpene biosynthesis in tomato.
引用
收藏
页码:2988 / 3005
页数:18
相关论文
共 77 条
[1]   CRISPR provides acquired resistance against viruses in prokaryotes [J].
Barrangou, Rodolphe ;
Fremaux, Christophe ;
Deveau, Helene ;
Richards, Melissa ;
Boyaval, Patrick ;
Moineau, Sylvain ;
Romero, Dennis A. ;
Horvath, Philippe .
SCIENCE, 2007, 315 (5819) :1709-1712
[2]   The development of type VI glandular trichomes in the cultivated tomato Solanum lycopersicum and a related wild species S. habrochaites [J].
Bergau, Nick ;
Bennewitz, Stefan ;
Syrowatka, Frank ;
Hause, Gerd ;
Tissier, Alain .
BMC PLANT BIOLOGY, 2015, 15
[3]   Divergent Regulation of Terpenoid Metabolism in the Trichomes of Wild and Cultivated Tomato Species [J].
Besser, Katrin ;
Harper, Andrea ;
Welsby, Nicholas ;
Schauvinhold, Ines ;
Slocombe, Stephen ;
Li, Yi ;
Dixon, Richard A. ;
Broun, Pierre .
PLANT PHYSIOLOGY, 2009, 149 (01) :499-514
[4]   RNA-seq discovery, functional characterization, and comparison of sesquiterpene synthases from Solanum lycopersicum and Solanum habrochaites trichomes [J].
Bleeker, Petra M. ;
Spyropoulou, Eleni A. ;
Diergaarde, Paul J. ;
Volpin, Hanne ;
De Both, Michiel T. J. ;
Zerbe, Philipp ;
Bohlmann, Joerg ;
Falara, Vasiliki ;
Matsuba, Yuki ;
Pichersky, Eran ;
Haring, Michel A. ;
Schuurink, Robert C. .
PLANT MOLECULAR BIOLOGY, 2011, 77 (4-5) :323-336
[5]   Jasmonic Acid and Its Precursor 12-Oxophytodienoic Acid Control Different Aspects of Constitutive and Induced Herbivore Defenses in Tomato [J].
Bosch, Marko ;
Wright, Louwrance P. ;
Gershenzon, Jonathan ;
Wasternack, Claus ;
Hause, Bettina ;
Schaller, Andreas ;
Stintzi, Annick .
PLANT PHYSIOLOGY, 2014, 166 (01) :396-U574
[6]   Methyl jasmonate application induces increased densities of glandular trichomes on tomato, Lycopersicon esculentum [J].
Boughton, AJ ;
Hoover, K ;
Felton, GW .
JOURNAL OF CHEMICAL ECOLOGY, 2005, 31 (09) :2211-2216
[7]   Reassess the t Test: Interact with All Your Data via ANOVA [J].
Brady, Siobhan M. ;
Burow, Meike ;
Busch, Wolfgang ;
Carlborg, Orjan ;
Denby, Katherine J. ;
Glazebrook, Jane ;
Hamilton, Eric S. ;
Harmer, Stacey L. ;
Haswell, Elizabeth S. ;
Maloof, Julin N. ;
Springer, Nathan M. ;
Kliebenstein, Daniel J. .
PLANT CELL, 2015, 27 (08) :2088-2094
[8]   Hair, encoding a single C2H2 zinc-finger protein, regulates multicellular trichome formation in tomato [J].
Chang, Jiang ;
Yu, Ting ;
Yang, Qihong ;
Li, Changxing ;
Xiong, Cheng ;
Gao, Shenghua ;
Xie, Qingmin ;
Zheng, Fangyan ;
Li, Hanxia ;
Tian, Zhendong ;
Yang, Changxian ;
Ye, Zhibiao .
PLANT JOURNAL, 2018, 96 (01) :90-102
[9]   Fine mapping of the dialytic gene that controls multicellular trichome formation and stamen development in tomato [J].
Chang, Jiang ;
Yu, Ting ;
Gao, Shenghua ;
Xiong, Cheng ;
Xie, Qingmin ;
Li, Hanxia ;
Ye, Zhibiao ;
Yang, Changxian .
THEORETICAL AND APPLIED GENETICS, 2016, 129 (08) :1531-1539
[10]   Tomato transformation and transgenic plant production [J].
Cortina, C ;
Culiáñez-Macià, FA .
PLANT CELL TISSUE AND ORGAN CULTURE, 2004, 76 (03) :269-275