SONODYNAMIC EFFECT OF AN ANTI-INFLAMMATORY AGENT - EMODIN ON MACROPHAGES

被引：36

作者：

Gao, Qianping ^{[2
,3
]}

Wang, Fengping

Guo, Shuyuan

Li, Jingyi ^{[2
]}

Zhu, Bidan ^{[2
]}

Cheng, Jiali

Jin, Yinghua

Li, Bo

Wang, Huan ^{[3
]}

Shi, Sa ^{[2
]}

Gao, Qiang ^{[4
]}

Zhang, Zhiguo ^{[4
]}

Cao, Wenwu ^{[4
,5
]}

Tian, Ye ^{[1
,2
]}

机构：

[1] Harbin Med Coll, Dept Cardiol, Affiliated Hosp 2, Harbin 150086, Peoples R China

[2] Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R China

[3] Harbin Med Coll, Affiliated Hosp 1, Harbin 150086, Peoples R China

[4] Harbin Med Coll, Dept Phys, Harbin 150086, Peoples R China

[5] Penn State Univ, Mat Res Inst, University Pk, PA 16802 USA

来源：

ULTRASOUND IN MEDICINE AND BIOLOGY | 2011年 / 37卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Atherosclerosis; Inflammation; Macrophage; Emodin; Sonodynamic therapy; ASCITES-CARCINOMA CELLS; PHOTODYNAMIC THERAPY; PROTOPORPHYRIN IX; IN-VITRO; ATHEROSCLEROTIC PLAQUES; SCAVENGER RECEPTOR; INDUCED APOPTOSIS; DAMAGE; STABILIZATION; CYTOSKELETON;

D O I：

10.1016/j.ultrasmedbio.2011.05.846

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

Emodin has been used as an anti-inflammatory agent and inflammation is a crucial feature of atherosclerosis. Here, we investigated the sonodynamic effect of emodin on macrophages, the pivotal inflammatory cells in atherosclerotic plaque. THP-1 derived macrophages were cultured with emodin and exposed to ultrasound. Six hours later, unlike the cells treated for 5 and 10 min, the viability of cells treated for 15 min decreased significantly and the cells showed typical apoptotic chromatin fragmentation. The percentage of apoptotic and necrotic cells in the sonodynamic therapy (SDT) group was higher than that in the ultrasound group. Two hours after treatment for 15 min, the cytoskeleton lost its original features as the filaments dispersed and the cytoskeletal proteins aggregated. The percentage of cells with disturbed cytoskeletal filaments in the SDT group was higher than that in the ultrasound group. These results suggest emodin has a sonodynamic effect on macrophages and might be used as a novel sonosensitizer for SDT for atherosclerosis. (E-mail: dzk@psu.edu and yetian@ems.hrbmu.edu.cn) (C) 2011 Published by Elsevier Inc on behalf of World Federation for Ultrasound in Medicine & Biology.

引用

页码：1478 / 1485

页数：8

共 29 条

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