The Impact of Curcumin on Bone Osteogenic Promotion of MC3T3 Cells under High Glucose Conditions and Enhanced Bone Formation in Diabetic Mice

被引:16
作者
He, Jia [1 ]
Yang, Xiaofeng [1 ]
Liu, Fan [1 ]
Li, Duo [1 ]
Zheng, Bowen [1 ]
Abdullah, Adil Othman [2 ,3 ]
Liu, Yi [1 ]
机构
[1] China Med Univ, Liaoning Prov Key Lab Oral Dis, Dept Orthodont, Sch Stomatol, Shenyang 110002, Peoples R China
[2] China Med Univ, Liaoning Prov Key Lab Oral Dis, Sch Stomatol, Shenyang 110002, Peoples R China
[3] Erbil Polytech Univ, Prosthodont Dept, Erbil 44001, Kurdistan Regio, Iraq
关键词
curcumin; high glucose; osteogenesis; bone formation; diabetic osteoporosis; OSTEOBLAST-LIKE CELLS; EXPERIMENTAL PERIODONTITIS; DIFFERENTIATION; TYPE-1; MINERALIZATION; PROLIFERATION; OSTEOPOROSIS; MELLITUS;
D O I
10.3390/coatings10030258
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Diabetic osteoporosis (DOP) is characterized by impaired bone microstructure and reduced bone density resulting from high glucose levels. Curcumin (CURC) is extensively applied in the treatment of inflammation-associated diseases. However, the effect of curcumin on bone metabolism in diabetic osteoporosis is unclear. Therefore, this study investigated the optimal concentration of curcumin on enhancing osteogenesis in diabetic osteoporosis. Osteoblasts were treated with a high or low concentration of curcumin under a series of concentrations of high-glucose conditions. Type 2 diabetic mice were intervened with curcumin. Cell proliferation, apoptosis, and osteogenesis-related gene expressions were evaluated by CCK-8, flow cytometry, and real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Bone formation was evaluated by histological staining. The findings revealed that curcumin suppressed apoptosis and enhanced proliferation and osteogenesis-related gene expressions of osteoblasts under high glucose concentrations (p < 0.05). The histological sections displayed reduced bone destruction and increased the growth rate of trabecular bone and the bone density of diabetic mice treated with curcumin, compared to diabetic mice. These results showed that curcumin could reverse the harmful effects of diabetic osteoporosis in a dose-dependent manner, and 10 mu mol/L was regarded as the optimal concentration, which supports the potential use of curcumin for bone regeneration under high glucose concentrations.
引用
收藏
页数:11
相关论文
共 36 条
[1]   The effect of diabetes mellitus on rat mandibular bone formation and microarchitecture [J].
Abbassy, Mona A. ;
Watari, Ippei ;
Soma, Kunimichi .
EUROPEAN JOURNAL OF ORAL SCIENCES, 2010, 118 (04) :364-369
[2]  
Alessandra G.M., 2020, INT IMMUNOPHARMACOL, V81
[3]  
Anita B., 2018, MEDICINA, V54, P14
[4]   Modulatory effect of curcumin analogs on the activation of metalloproteinases in human periodontal stem cells [J].
Bosca, Adina B. ;
Ilea, Aranka ;
Soritau, Olga ;
Tatomir, Corina ;
Miklasova, Natalia ;
Parvu, Alina E. ;
Mihu, Carmen M. ;
Melincovici, Carmen S. ;
Fischer-Fodor, Eva .
EUROPEAN JOURNAL OF ORAL SCIENCES, 2019, 127 (04) :304-312
[5]   Antiplasmodial Activity and Toxicological Assessment of Curcumin PLGA-Encapsulated Nanoparticles [J].
Busari, Zulaikha A. ;
Dauda, Kabiru A. ;
Morenikeji, Olajumoke A. ;
Afolayan, Funmilayo ;
Oyeyemi, Oyetunde T. ;
Meena, Jairam ;
Sahu, Debasis ;
Panda, Amulya K. .
FRONTIERS IN PHARMACOLOGY, 2017, 8
[6]   Short communication: selective cytotoxicity of curcumin on osteosarcoma cells compared to healthy osteoblasts [J].
Chang, Run ;
Sun, Linlin ;
Webster, Thomas J. .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2014, 9 :461-465
[7]   Effect of curcumin on bone tissue in the diabetic rat: repair of peri-implant and critical-sized defects [J].
Cirano, F. R. ;
Pimentel, S. P. ;
Casati, M. Z. ;
Correa, M. G. ;
Pino, D. S. ;
Messora, M. R. ;
Silva, P. H. F. ;
Ribeiro, F. V. .
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2018, 47 (11) :1495-1503
[8]   Delayed osteoblast differentiation and altered inflammatory response around implants placed in incisor sockets of type 2 diabetic rats [J].
Colombo, John S. ;
Balani, Deepak ;
Sloan, Alastair J. ;
Crean, St John ;
Okazaki, Joji ;
Waddington, Rachel J. .
CLINICAL ORAL IMPLANTS RESEARCH, 2011, 22 (06) :578-586
[9]   Systemic treatment with resveratrol and/or curcumin reduces the progression of experimental periodontitis in rats [J].
Correa, M. G. ;
Pires, P. R. ;
Ribeiro, F. V. ;
Pimentel, S. Z. ;
Casarin, R. C. V. ;
Cirano, F. R. ;
Tenenbaum, H. T. ;
Casati, M. Z. .
JOURNAL OF PERIODONTAL RESEARCH, 2017, 52 (02) :201-209
[10]   Biological Silicon Stimulates Collagen Type 1 and Osteocalcin Synthesis in Human Osteoblast-Like Cells Through the BMP-2/Smad/RUNX2 Signaling Pathway [J].
Dong, Meng ;
Jiao, Guangjun ;
Liu, Haichun ;
Wu, Wenliang ;
Li, Shangzhi ;
Wang, Qingshi ;
Xu, Daxia ;
Li, Xiaofeng ;
Liu, Huan ;
Chen, Yunzhen .
BIOLOGICAL TRACE ELEMENT RESEARCH, 2016, 173 (02) :306-315