Body fluid cellular analysis using the Sysmex XN-2000 automatic hematology analyzer: focusing on malignant samples

被引：41
作者：
Cho, Y. -U. [1 ,2 ]
Chi, H. -S. [1 ,2 ]
Park, S. H. [3 ]
Jang, S. [1 ,2 ]
Kim, Y. -J. [1 ,2 ]
Park, C. -J. [1 ,2 ]
机构：
[1] Univ Ulsan, Coll Med, Dept Lab Med, Seoul 138736, South Korea
[2] Asan Med Ctr, Seoul, South Korea
[3] Pusan Natl Univ, Sch Med, Pusan Natl Univ Hosp, Dept Lab Med, Busan, South Korea
来源：
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY | 2015年 / 37卷 / 03期
关键词：
Body fluid; automatic hematology analyzer; Sysmex XN; manual microscopy; malignant sample; HF-body fluid cells; CEREBROSPINAL-FLUID; BLOOD-CELLS; PERFORMANCE EVALUATION; XE-5000; MODE;
D O I：
10.1111/ijlh.12292
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
IntroductionThe majority of previous studies on body fluid (BF) mode of automatic hematology analyzer used nonmalignant BF samples. Here, we evaluated the BF mode on the recently launched Sysmex XN for counting blood cells, especially for malignant samples. MethodsA total of 405 BF specimens including 125 malignant samples were analyzed using both the automated method and manual microscopy. ResultsIn non-cerebrospinal fluids (CSF) samples, there was an agreement between two methods for WBC, RBC, polymorphonuclear, and mononuclear cell counts (R-2=0.96, 0.94, 0.88, and 0.88, respectively). CSF samples showed slightly poorer correlations than other fluids. Exclusion of malignant samples significantly improved correlations in non-CSF samples, but not in CSF samples. High fluorescence-BF (HF-BF) cells were identified significantly more frequently in malignant samples compared to benign samples (17.8 and 4.15/100 WBC, respectively; P<0.001). Receiver operating characteristic curve analysis demonstrated an HF-BF cell AUC of 0.791 using a cutoff value of 6.9/100 WBC for detecting malignant samples. ConclusionThe BF mode on the Sysmex XN could be an alternative method for the manual counts in the BF analysis with a few drawbacks. However, if a concentration of HF-BF cells is greater than the given threshold, microscopic examination should be subsequently performed.
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页码：346 / 356
页数：11
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