Pseudogene PDIA3P1 promotes cell proliferation, migration and invasion, and suppresses apoptosis in hepatocellular carcinoma by regulating the p53 pathway

被引：56

作者：

Kong, Yang ^{[1
,4
]}

Zhang, Lufei ^{[1
,4
]}

Huang, Yu ^{[1
,4
]}

He, Tianyu ^{[1
,4
]}

Zhang, Linshi ^{[1
,4
]}

Zhao, Xinyi ^{[2
,3
]}

Zhou, Xiaohu ^{[1
,4
]}

Zhou, Dongkai ^{[1
,4
]}

Yan, Yingcai ^{[1
,4
]}

Zhou, Jiarong ^{[1
,4
]}

Xie, Haiyang ^{[5
]}

Zhou, Lin ^{[5
]}

Zheng, Shusen ^{[1
,4
,5
]}

Wang, Weilin ^{[1
,2
,3
,4
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Key Lab Precis Diag & Treatment Hepatobiliary & P, Hangzhou, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, State Key Lab, Hangzhou, Zhejiang, Peoples R China

[3] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China

[4] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Div Hepatobiliary & Pancreat Surg, Hangzhou, Zhejiang, Peoples R China

[5] Minist Publ Hlth, Key Lab Combined Multiorgan Transplantat, Hangzhou, Zhejiang, Peoples R China

来源：

CANCER LETTERS | 2017年 / 407卷

基金：

中国国家自然科学基金;

关键词：

Long noncoding RNA; Pseudogene; Hepatocellular carcinoma; p53; LONG NONCODING RNAS; CANCER STATISTICS; METASTASIS; PROGRESSION; EXPRESSION; TARGET; P21;

D O I：

10.1016/j.canlet.2017.07.031

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Pseudogenes are a subclass of long non-coding (Inc) RNAs that arose from protein-coding genes, but have lost the ability to produce proteins. Pseudogenes play an important role in the pathogenesis of various tumors; however, the role of pseudogenes in hepatocellular carcinoma (HCC) is poorly understood. In this study, we investigated a novel pseudogene, PDIA3P1, which was upregulated in HCC tissues compared with paired normal adjacent tissues. The expression of PDIA3P1 was significantly correlated with tumor size, metastasis, TNM stage, and overall stage. Knockdown of PDIA3Pldecreased proliferation, migration, and invasion of HCC cells. PDIA3P1 knockdown also promoted cell apoptosis and inhibited tumor growth in vivo. We performed a GeneChip assay to investigate the underlying mechanism of PDIA3P1 action on biological function, and our results suggested that PDIA3P1 may promote proliferation and inhibit apoptosis of liver cancer cells by inhibiting the p53 pathway. Thus, our data suggest that PDIA3P1 acts as an oncogene in HCC and could be a potential prognostic marker and therapeutic target for HCC. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：76 / 83

页数：8

共 38 条

[1] p21 in cancer: intricate networks and multiple activities [J].