Evaluation of VITEK 2, MicroScan, and Phoenix for identification of clinical isolates and reference strains

被引:39
作者
Jin, Won-Young [1 ]
Jang, Sook-Jin [1 ,2 ]
Lee, Min-Jung [2 ]
Park, Geon [1 ]
Kim, Min-Jung [3 ]
Kook, Joong-Ki [3 ]
Kim, Dong-Min [4 ]
Moon, Dae-Soo [1 ]
Park, Young-Jin [1 ]
机构
[1] Chosun Univ, Sch Med, Dept Lab Med, Kwangju, South Korea
[2] Chosun Univ, Sch Med, Res Ctr Resistant Cells, Kwangju, South Korea
[3] Chosun Univ, Coll Dent, Dept Oral Biochem, Kwangju, South Korea
[4] Chosun Univ, Sch Med, Dept Internal Med, Kwangju, South Korea
关键词
Bacteria; Identification; Equipment; Evaluation studies; Task performance and analysis; SYSTEM; ENTEROBACTERIACEAE; WALKAWAY; BACILLI; RODS; CARD;
D O I
10.1016/j.diagmicrobio.2011.04.013
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
To compare the identification accuracies of VITEK 2 (bioMerieux), MicroScan (Siemens Healthcare), and Phoenix (Becton Dickinson), microbial identification was performed on 160 clinical isolates and 50 reference strains on each of these 3 systems, using the appropriate identification kit provided by each system. Of the 142 clinical isolates that were identified at the species level, VITEK 2, MicroScan, and Phoenix correctly identified 93.7%, 82.4%, and 93.0%, and incorrectly identified 2.1%, 7.0%, and 0%, respectively. In the reference strain tests, VITEK 2, MicroScan, and Phoenix correctly identified 55.3%, 54.4%, and 78.0% of the reference strains at the species level and incorrectly identified 10.6%, 13.0%, and 6.0% of the reference strains, respectively. In conclusion, the identification rate of VITEK 2, Phoenix, and MicroScan was high or acceptable on clinical isolates. Phoenix showed a significantly higher performance than VITEK 2 or MicroScan in identifying the reference strains. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:442 / 447
页数:6
相关论文
共 15 条
[1]   16S rRNA gene sequencing versus the API 20 NE system and the VITEK 2 ID-GNB card for identification of nonfermenting gram-negative bacteria in the clinical laboratory [J].
Bosshard, PP ;
Zbinden, R ;
Abels, S ;
Böddinghaus, B ;
Altwegg, M ;
Böttger, EC .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (04) :1359-1366
[2]   Evaluation of the BD phoenix automated microbiology system for identification and antimicrobial susceptibility testing of Enterobacteriaceae [J].
Carroll, Karen C. ;
Glanz, Brian D. ;
Borek, Anita P. ;
Burger, Chad ;
Bhally, Hasan S. ;
Henciak, Susan ;
Flayhart, Diane .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (10) :3506-3509
[3]   Evaluation of the automated phoenix system for potential routine use in the clinical microbiology laboratory [J].
Donay, JL ;
Mathieu, D ;
Fernandes, P ;
Prégermain, C ;
Bruel, P ;
Wargnier, A ;
Casin, I ;
Weill, FX ;
Lagrange, PH ;
Herrmann, JL .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) :1542-1546
[4]   Analysis of the comparative workflow and performance characteristics of the VITEK 2 and Phoenix systems [J].
Eigner, U ;
Schmid, A ;
Wild, U ;
Bertsch, D ;
Fahr, AM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) :3829-3834
[5]   Comparison of the phenotyping methods ID 32E and VITEK 2 compact GN with 16S rRNA gene sequencing for the identification of Enterobacter sakazakii [J].
Fanjat, Nadege ;
Leclercq, Alexandre ;
Joosten, Han ;
Robichon, Denis .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) :2048-2050
[6]   Evaluation of an automated system for identification of Enterobacteriaceae and nonfermenting bacilli [J].
Jossart, MF ;
Courcol, RJ .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1999, 18 (12) :902-907
[7]   Evaluation of the VITEK 2 system for the identification and susceptibility testing of three species of nonfermenting gram-negative rods frequently isolated from clinical samples [J].
Joyanes, P ;
Conejo, MD ;
Martínez-Martínez, L ;
Perea, EJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (09) :3247-3253
[8]   Distinct antimicrobial resistance patterns and antimicrobial resistance-harboring genes according to genomic species of Acinetobacter isolates [J].
Lim, Yu Mi ;
Shin, Kyeong Seob ;
Kim, Jungmin .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (03) :902-905
[9]   Evaluation of the VITEK 2 system for rapid direct identification and susceptibility testing of gram-negative bacilli from positive blood cultures [J].
Ling, TKW ;
Liu, ZK ;
Cheng, AFB .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4705-4707
[10]   Comparison of 16S rRNA sequencing with conventional and commercial phenotypic techniques for identification of enterococci from the marine environment [J].
Moore, D. F. ;
Zhowandai, M. H. ;
Ferguson, D. M. ;
Mcgee, C. ;
Mott, J. B. ;
Stewart, J. C. .
JOURNAL OF APPLIED MICROBIOLOGY, 2006, 100 (06) :1272-1281