Osteoprogenitor Cells from Bone Marrow and Cortical Bone: Understanding How the Environment Affects Their Fate

被引:31
作者
Corradetti, Bruna [1 ,2 ]
Taraballi, Francesca [1 ,3 ]
Powell, Sebastian [1 ]
Sung, David [1 ]
Minardi, Silvia [1 ,4 ]
Ferrari, Mauro [1 ]
Weiner, Bradley K. [1 ,5 ]
Tasciotti, Ennio [1 ]
机构
[1] Houston Methodist Res Inst, Dept Nanomed, Houston, TX 77030 USA
[2] Univ Politecn Marche, Dept Life & Environm Sci, Ancona, Italy
[3] Fdn IRCCS Policlin San Matteo, Pain Therapy Serv, Pavia, Italy
[4] Natl Res Council Italy, Inst Sci & Technol Ceram, Bioceram & Biohybrid Mat, Faenza, Italy
[5] Houston Methodist Hosp, Dept Orthoped Surg, Houston, TX USA
关键词
MESENCHYMAL STEM-CELLS; GROWTH-FACTOR RECEPTOR; VERSUS-HOST-DISEASE; STROMAL CELLS; PROGENITOR CELLS; TRABECULAR BONE; AMNIOTIC-FLUID; IN-VIVO; ADULT; MULTIPOTENT;
D O I
10.1089/scd.2014.0351
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Bone is a dynamic organ where skeletal progenitors and hematopoietic cells share and compete for space. Presumptive mesenchymal stem cells (MSC) have been identified and harvested from the bone marrow (BM-MSC) and cortical bone fragments (CBF-MSC). In this study, we demonstrate that despite the cells sharing a common ancestor, the differences in the structural properties of the resident tissues affect cell behavior and prime them to react differently to stimuli. Similarly to the bone marrow, the cortical portion of the bone contains a unique subset of cells that stains positively for the common MSC-associated markers. These cells display different multipotent differentiation capability, clonogenic expansion, and immunosuppressive potential. In particular, when compared with BM-MSC, CBF-MSC are bigger in size, show a lower proliferation rate at early passages, have a greater commitment toward the osteogenic lineage, constitutively produce nitric oxide as a mediator for bone remodeling, and more readily respond to proinflammatory cytokines. Our data suggest that the effect of the tissue's microenvironment makes the CBF-MSC a superior candidate in the development of new strategies for bone repair.
引用
收藏
页码:1112 / 1123
页数:12
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