Background: Tooth brushing and other mechanical cleaning procedures are considered the most reliable methods of effective plaque removal, which is essential for prevention of periodontal diseases. Objective: To investigate the relationship between oral hygiene practices, socioeconomic status and gingival health in a group of Nigerian children. Methods: Our study population consisted of 242 randomly selected school children in Ile-Ife, Nigeria. Participants completed a questionnaire on oral hygiene measures, their gingival health was assessed using oral hygiene index (OHI), plaque index (PI) and index of gingival inflammation (GI). Results: Toothbrushing once daily was the most common practice (52.1%). Toothbrush with a fluoride-containing toothpaste was the most common tooth cleaning aid, while the up and down brushing technique was predominantly used. There was no statistically significant gender difference in toothbrushing frequency; however, significant gender differences were observed in PI and OHI scores (P < 0.05). Gingivitis was absent in 28.9% of the children,, while 50.8% had mild, 13.6% moderate and 6.6% severe gingivitis. There was a weak but significant negative correlation between oral hygiene frequency and GI (P < 0.05). Socioeconomic status had no relationship with oral hygiene frequency, however it had low but significant correlation with OHI and GI (P < 0.05). A logistic regression analysis of the predictors on gingivitis showed that male gender and medium textured toothbrush had significant association with gingival health. Conclusions: Gingival health was influenced by gender, socioeconomic status, oral hygiene frequency and toothbrush texture. Motivation to apply instructions given on oral health care and regular reinforcement are essential.