The role of hemocytes, serine protease inhibitors and pathogen-associated patterns in prophenoloxidase activation in the desert locust, Schistocerca gregaria

被引：32
作者：
Franssens, Vanessa [1 ]
Simonet, Gert [1 ]
Breugelmans, Bert [1 ]
Van Soest, Sofie [1 ]
Van Hoef, Vincent [1 ]
Broeck, Jozef Vanden [1 ]
机构：
[1] Katholieke Univ Leuven, Inst Zool, Dept Anim Physiol & Neurobiol, Lab Dev Physiol Genom & Prot, B-3000 Louvain, Belgium
来源：
PEPTIDES | 2008年 / 29卷 / 02期
关键词：
innate immunity; phenoloxidase; peptide; serine protease inhibitor; insect; hemolymph;
D O I：
10.1016/j.peptides.2007.07.032
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The prophenoloxidase-activating system is an important component of the innate immune response of insects, involved in wound healing and melanotic encapsulation. In this paper we show that in the desert locust, Schistocerca gregaria, hemocytes, challenged with microbial elicitors, are indispensable for the limited proteolytic activation of prophenoloxidase (proPO) in plasma. in addition, we assessed the influence of serine protease inhibitors on the induction of PO-activity in plasma. While soybean Bowman-Birk inhibitor (SBBI) inhibited the PO activation by laminarin-treated hemocytes, the endogenous pacifastin-related inhibitors, SGPI-1 (S. gregaria pacifastin-related inhibitor-1) and SGPI-2 did not affect the PO-activity under similar conditions. On the other hand, real-time PCR analysis revealed that the transcripts, encoding SGPI-1-3, were more abundant in the fat body of immune challenged animals, as compared to control animals. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：235 / 241
页数：7
相关论文
共 23 条
[1]   THE EFFECT OF ENDOGENEOUS PROTEINASE-INHIBITORS ON THE PROPHENOLOXIDASE ACTIVATING ENZYME, A SERINE PROTEINASE FROM CRAYFISH HEMOCYTES [J].
ASPAN, A ;
HALL, M ;
SODERHALL, K .
INSECT BIOCHEMISTRY, 1990, 20 (05) :485-&
[2]   INSECT IMMUNITY - 2 PROTEINASE-INHIBITORS FROM HEMOLYMPH OF LOCUSTA-MIGRATORIA [J].
BOIGEGRAIN, RA ;
MATTRAS, H ;
BREHELIN, M ;
PAROUTAUD, P ;
COLETTIPREVIERO, MA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 189 (02) :790-793
[3]   PURIFICATION OF A PROTEASE INHIBITOR WHICH CONTROLS PROPHENOLOXIDASE ACTIVATION IN HEMOLYMPH OF LOCUSTA-MIGRATORIA (INSECTA) [J].
BREHELIN, M ;
BOIGEGRAIN, RA ;
DRIF, L ;
COLETTIPREVIERO, MA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 179 (02) :841-846
[4]   Cloning of two cDNAs encoding three small serine protease inhibiting peptides from the desert locust Schistocerca gregaria and analysis of tissue-dependent and stage-dependent expression [J].
Broeck, JV ;
Chiou, SJ ;
Schoofs, L ;
Hamdaoui, A ;
Vandenbussche, F ;
Simonet, G ;
Wataleb, S ;
De Loof, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 254 (01) :90-95
[5]   The prophenoloxidase-activating system in invertebrates [J].
Cerenius, L ;
Söderhäll, K .
IMMUNOLOGICAL REVIEWS, 2004, 198 :116-126
[6]   Quantitative real-time RT-PCR analysis in desert locusts reveals phase dependent differences in neuroparsin transcript levels [J].
Claeys, I ;
Simonet, G ;
Breugelmans, B ;
Van Soest, S ;
Franssens, V ;
Sas, F ;
De Loof, A ;
Vanden Broeck, J .
INSECT MOLECULAR BIOLOGY, 2005, 14 (04) :415-422
[7]   cDNA cloning and transcript distribution of two novel members of the neuroparsin family in the desert locust, Schistocerca gregaria [J].
Claeys, I ;
Simonet, G ;
Van Loy, T ;
De Loof, A ;
Vanden Broeck, J .
INSECT MOLECULAR BIOLOGY, 2003, 12 (05) :473-481
[8]   Adipokinetic hormone enhances nodule formation and phenoloxidase activation in adult locusts injected with bacterial lipopolysaccharide [J].
Goldsworthy, G ;
Chandrakant, S ;
Opoku-Ware, K .
JOURNAL OF INSECT PHYSIOLOGY, 2003, 49 (08) :795-803
[9]   Adipokinetic hormone enhances laminarin and bacterial lipopolysaccharide-induced activation of the prophenoloxidase cascade in the African migratory locust, Locusta migratoria [J].
Goldsworthy, G ;
Opoku-Ware, K ;
Mullen, L .
JOURNAL OF INSECT PHYSIOLOGY, 2002, 48 (06) :601-608
[10]   Purification and characterization of a group of five novel peptide serine protease inhibitors from ovaries of the desert locust, Schistocerca gregaria [J].
Hamdaoui, A ;
Wataleb, S ;
Devreese, B ;
Chiou, SJ ;
Vanden Broeck, J ;
Van Beeumen, J ;
De Loof, A ;
Schoofs, L .
FEBS LETTERS, 1998, 422 (01) :74-78
123