Tissue-specific modulation of rat glucagon receptor mRNA by thyroid status

被引：10

作者：

Morales, A ^{[1
]}

Lachuer, J ^{[1
]}

Duchamp, C ^{[1
]}

Vera, N ^{[1
]}

Georges, B ^{[1
]}

Cohen-Adad, F ^{[1
]}

Moulin, C ^{[1
]}

Barré, H ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, UMR 5578,Fac Sci, Lab Physiol Regulat Energet Cellulaires & Mol, F-69622 Villeurbanne, France

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1998年 / 144卷 / 1-2期

关键词：

semi-quantitative RT-PCR; liver; brown adipose tissue; white adipose tissue; brain; thyroid hormone; rat;

D O I：

10.1016/S0303-7207(98)00150-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The influence of thyroid status on glucagon receptor mRNA levels was investigated in rats using a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Glucagon receptor mRNA was detected in liver, brown and white adipose tissues (BAT and WAT) and brain. In BAT and WAT, pharmacologically-induced moderate hypothyroidism resulted in a marked reduction in the relative abundance of glucagon receptor mRNA. Short-term treatment of hypothyroid rats with exogenous 3,3',5'-triiodo-L-thyronine (T-3), resulting in a marked hyperthyroidism, reversed the phenomenon in BAT while the reversal was only partial in WAT. In the liver, there was no significant effect of mild hypothyroidism while there was a positive effect of hyperthyroidism. In brain, the relative tissue abundance of glucagon receptor mRNA was not affected by the large changes in plasma T-3. The present results therefore indicate that thyroid status may modulate the relative abundance of glucagon receptor mRNA in a tissue-specific manner. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：71 / 81

页数：11

共 31 条

[1] REGULATION OF GLUCAGON RECEPTOR MESSENGER-RNA IN CULTURED PRIMARY RAT HEPATOCYTES BY GLUCOSE AND CAMP [J].