Immobilization of thrombocytes on PCL nanofibres enhances chondrocyte proliferation in vitro

被引：28
作者：
Jakubova, R. [1 ,2 ]
Mickova, A. [1 ]
Buzgo, M. [1 ,5 ]
Rampichova, M.
Prosecka, E. [1 ]
Tvrdik, D. [3 ,4 ]
Amler, E. [1 ]
机构：
[1] Charles Univ Prague, Fac Med 2, Inst Biophys, Prague 15006 5, Motol, Czech Republic
[2] Acad Sci Czech Republ, Inst Expt Med, Lab Tissue Engn, Vvi, Prague, Czech Republic
[3] Charles Univ Prague, Fac Med 1, Inst Pathol, Prague 15006 5, Motol, Czech Republic
[4] Charles Univ Prague, Gen Teaching Hosp, Prague 15006 5, Motol, Czech Republic
[5] Charles Univ Prague, Fac Sci, Dept Cell Biol, Prague 15006 5, Motol, Czech Republic
来源：
CELL PROLIFERATION | 2011年 / 44卷 / 02期
关键词：
PLATELET-RICH PLASMA; FIBROCYTES; EXPANSION; MATRIX; CELLS;
D O I：
10.1111/j.1365-2184.2011.00737.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Objectives: The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention. Materials and methods: We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocyte-coated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds. Results: Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs. Conclusions: These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.
引用
收藏
页码：183 / 191
页数：9
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