Isolation and characterization of canine tumor endothelial cells

被引：1

作者：

Izumi, Yusuke ^{[1
]}

Hoshino, Yuki ^{[2
]}

Hosoya, Kenji ^{[1
]}

Takagi, Satoshi ^{[2
]}

Okumura, Masahiro ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Vet Med, Dept Vet Clin Sci, Lab Vet Surg, Sapporo, Hokkaido 0600818, Japan

[2] Hokkaido Univ, Grad Sch Vet Med, Vet Teaching Hosp, Sapporo, Hokkaido 0600818, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2015年 / 77卷 / 03期

关键词：

angiogenesis; canine; tumor endothelial cell; RESISTANCE; CARCINOMA; GROWTH; CANCER; MARKER; CXCR7;

D O I：

10.1292/jvms.14-0347

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The present study involved the isolation and characterization of canine tumor endothelial cells (TECs) from 2 malignancies. TECs were isolated using magnetic cell sorting following FITC labeling with UEA1 lectin, and they were characterized by measuring genetic and histopathological endothelial markers. Isolated TECs exhibited a cobblestone-like morphology and expressed both vascular endothelial growth factor receptor 2 (VEGFR2) and Von Willebrand factor (vWF). Further, both TECs and tumor cells derived from a seminoma exhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression. However, CXCR7 expression was not detected in TECs and tumor cells derived from a hepatocellular carcinoma. Understanding TEC specific traits may be important in the development of more efficacious anti-angiogenic therapies that do not induce adverse effects.

引用

页码：359 / 363

页数：5

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