In vitro activity of 1H-phenalen-1-one derivatives against Leishmania spp. and evidence of programmed cell death

被引：12

作者：

Lopez-Arencibia, Atteneri ^{[1
,2
]}

Reyes-Batlle, Maria ^{[1
]}

Freijo, Monica B. ^{[3
]}

Sifaoui, Ines ^{[1
]}

Bethencourt-Estrella, Carlos J. ^{[1
]}

Rizo-Liendo, Aitor ^{[1
]}

Chiboub, Olfa ^{[1
,4
]}

McNaughton-Smith, Grant ^{[5
]}

Lorenzo-Morales, Jacob ^{[1
,2
]}

Abad-Grillo, Teresa ^{[3
]}

Pinero, Jose E. ^{[1
,2
]}

机构：

[1] Univ La Laguna, Inst Univ Enfermedades Trop & Salud Publ Canarias, Avda Astrofis Fco Sanchez S-N, Tenerife 38203, Islas Canarias, Spain

[2] Univ La Laguna, Dept Obstet & Ginecol, Pediat Med Prevent & Salud Publ Toxicol Med Legal, Tenerife, Spain

[3] Univ La Laguna, Inst Univ Bioorgan Antonio Gonzalez, Dept Quim Organ, Avda Fco Sanchez 2, Tenerife 38206, Islas Canarias, Spain

[4] Univ Carthage, Lab Mat Mol & Applicat, La Marsa, Carthage, Tunisia

[5] Ctr Atlantico Med SA CEAMED SA, PCTT, Tenerife, Islas Canarias, Spain

来源：

PARASITES & VECTORS | 2019年 / 12卷 / 01期

关键词：

Leishmania donovani; Leishmania amazonensis; Phenalenones; Chemotherapy; Apoptosis-like; PHYTOALEXINS; METABOLITES; ACTIVATION; EFFICACY; THERAPY;

D O I：

10.1186/s13071-019-3854-4

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background The in vitro activity against Leishmania spp. of a novel group of compounds, phenalenone derivatives, is described in this study. Previous studies have shown that some phenalenones present leishmanicidal activity, and induce a decrease in the mitochondrial membrane potential in L. amazonensis parasites, so in order to elucidate the evidence of programmed cell death occurring inside the promastigote stage, different assays were performed in two different species of Leishmania. Methods We focused on the determination of the programmed cell death evidence by detecting the characteristic features of the apoptosis-like process, such as phosphatidylserine exposure, mitochondrial membrane potential, and chromatin condensation among others. Results The results showed that four molecules activated the apoptosis-like process in the parasite. All the signals observed were indicative of the death process that the parasites were undergoing. Conclusions The present results highlight the potential use of phenalenone derivatives against Leishmania species and further studies should be undertaken to establish them as novel leishmanicidal therapeutic agents.

引用

页数：10

共 40 条

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