The multiple antigen blot assay (MABA): a simple immunoenzymatic technique for simultaneous screening of multiple antigens

被引:37
作者
Noya, O
de Noya, BA
机构
[1] Cent Univ Venezuela, Secc Biohelmintiasis, Inst Trop Med, Caracas, Venezuela
[2] UCV, INH, Lab Estudios Malaria Malariol, Caracas, Venezuela
关键词
multiple dot-blot; antigen; peptides; screening; ELISA;
D O I
10.1016/S0165-2478(98)00055-8
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We describe a simple, reliable, reproducible and inexpensive technique termed multiple antigen blot assay (MABA) that permits the simultaneous screening of 28 different antigens based on a dot-blot ELISA methodology. Using an acrylic device (Miniblotter(R)) containing 28 parallel troughs, multiple antigens are distributed and immobilized onto a nitrocellulose membrane. Strips are then cut perpendicularly and exposed to immune serum. The reaction is detected with secondary antibodies conjugated to horseradish peroxidase, and developed by a chemiluminescent substrate, the results being recorded on film. Positive reactions to the different antigens are seen as small black squares on each strip. We have used this qualitative technique to screen synthetic peptides derived from native schistosomal and malarial proteins, using immune rabbit sera as the detection antibody. This system can also be used in the clinical laboratory according to a dipstick-based diagnostic format for different infectious and non-infectious diseases, and can be designed to detect either antibodies or circulating antigens. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:53 / 56
页数:4
相关论文
共 6 条
[1]   CROSS-BLOT AND CROSS-DOT SYSTEM - A HIGH-PERFORMANCE SYSTEM FOR THE DETECTION OF ANTIGEN-ANTIBODY COMPLEXES ON NITROCELLULOSE [J].
ALRIC, M ;
CHEYVIALLE, D ;
RENAUD, M .
ANALYTICAL BIOCHEMISTRY, 1986, 155 (02) :328-334
[3]   CHECKERBOARD IMMUNOBLOTTING (CBIB) - AN EFFICIENT, RAPID, AND SENSITIVE METHOD OF ASSAYING MULTIPLE ANTIGEN-ANTIBODY CROSS-REACTIVITIES [J].
KAZEMI, M ;
FINKELSTEIN, RA .
JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 128 (01) :143-146
[4]   SOLID PHASE PEPTIDE SYNTHESIS .1. SYNTHESIS OF A TETRAPEPTIDE [J].
MERRIFIELD, RB .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1963, 85 (14) :2149-&
[5]   HUMORAL IMMUNE-RESPONSE OF CHILDREN WITH CHRONIC SCHISTOSOMIASIS - ISOTYPE RECOGNITION OF ADULT WORM ANTIGENS [J].
NOYA, O ;
FERMIN, Z ;
DENOYA, BA ;
LOSADA, S ;
COLMENARES, C ;
HERMOSO, T .
PARASITE IMMUNOLOGY, 1995, 17 (06) :319-328
[6]   ENZYME IMMUNOASSAYS FOR PARASITIC DISEASES [J].
VOLLER, A ;
BARTLETT, A ;
BIDWELL, DE .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1976, 70 (02) :98-106