Rapid control of protein level in the apicomplexan Toxoplasma gondii

被引：159

作者：

Herm-Goetz, Angelika

Agop-Nersesian, Carolina

Muenter, Sylvia

Grimley, Joshua S.

Wandless, Thomas J.

Frischknecht, Friedrich

Meissner, Markus ^{[1
]}

机构：

[1] Univ Heidelberg Hosp, Inst Hyg, Dept Parasitol, D-69120 Heidelberg, Germany

[2] Stanford Univ, Dept Chem & Syst Biol, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 12期

关键词：

D O I：

10.1038/nmeth1134

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite.

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收藏

页码：1003 / U8

页数：4

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