Changes in low-molecular-weight thiol-disulphide redox couples are part of bread wheat seed germination and early seedling growth

被引:21
作者
Gerna, Davide [1 ]
Roach, Thomas [1 ]
Stoeggl, Wolfgang [1 ]
Wagner, Johanna [1 ]
Vaccino, Patrizia [2 ]
Limonta, Margherita [2 ]
Kranner, Ilse [1 ]
机构
[1] Univ Innsbruck, CMBI, Dept Bot, Sternwartestr 15, A-6020 Innsbruck, Austria
[2] Res Unit Cereal Select Continental Areas, Consiglio Ric Agr Analisi Econ Agraria, San Angelo Lodigiano, Italy
关键词
Cysteine; embryo; endosperm; glutathione; reactive oxygen species; Triticum aestivum L; PROGRAMMED CELL-DEATH; EXTRACELLULAR-SUPEROXIDE PRODUCTION; HYDROGEN-PEROXIDE; PROTEIN-SYNTHESIS; PLANT DEVELOPMENT; STORAGE PROTEINS; OXALATE OXIDASE; THIOREDOXIN-H; GLUTATHIONE HOMEOSTASIS; OXIDATIVE STRESS;
D O I
10.1080/10715762.2017.1338344
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tripeptide antioxidant glutathione (-l-glutamyl-l-cysteinyl-glycine; GSH) essentially contributes to thiol-disulphide conversions, which are involved in the control of seed development, germination, and seedling establishment. However, the relative contribution of GSH metabolism in different seed structures is not fully understood. We studied the GSH/glutathione disulphide (GSSG) redox couple and associated low-molecular-weight (LMW) thiols and disulphides related to GSH metabolism in bread wheat (Triticum aestivum L.) seeds, focussing on redox changes in the embryo and endosperm during germination. In dry seeds, GSH was the predominant LMW thiol and, 15h after the onset of imbibition, embryos of non-germinated seeds contained 12 times more LMW thiols than the endosperm. In germinated seeds, the embryo contained 17 and 11 times more LMW thiols than the endosperm after 15 and 48h, respectively. This resulted in the embryo having significantly more reducing half-cell reduction potentials of GSH/GSSG and cysteine (Cys)/cystine (CySS) redox couples (E-GSSG/2GSH and E-CySS/2Cys, respectively). Upon seed germination and early seedling growth, Cys and CySS concentrations significantly increased in both embryo and endosperm, progressively contributing to the cellular LMW thiol-disulphide redox environment (Ethiol-disulphide). The changes in E-CySS/2Cys could be related to the mobilisation of storage proteins in the endosperm during early seedling growth. We suggest that E-GSSG/2GSH and E-CySS/2Cys can be used as markers of the physiological and developmental stage of embryo and endosperm. We also present a model of interaction between LMW thiols and disulphides with hydrogen peroxide (H2O2) in redox regulation of bread wheat germination and early seedling growth.
引用
收藏
页码:568 / 581
页数:14
相关论文
共 74 条
  • [1] Bailly C, 2011, ANAL REACTIVE OXYGEN, P343
  • [2] From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology
    Bailly, Christophe
    El-Maarouf-Bouteau, Hayat
    Corbineau, Francoise
    [J]. COMPTES RENDUS BIOLOGIES, 2008, 331 (10) : 806 - 814
  • [3] Interaction between hydrogen peroxide and plant hormones during germination and the early growth of pea seedlings
    Barba-Espin, G.
    Diaz-Vivancos, P.
    Clemente-Moreno, M. J.
    Albacete, A.
    Faize, L.
    Faize, M.
    Perez-Alfocea, F.
    Hernandez, J. A.
    [J]. PLANT CELL AND ENVIRONMENT, 2010, 33 (06) : 981 - 994
  • [4] Alterations of the glutathione redox state improve apical meristem structure and somatic embryo quality in white spruce (Picea glauca)
    Belmonte, MF
    Donald, G
    Reid, DM
    Yeung, EC
    Stasolla, C
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 2005, 56 (419) : 2355 - 2364
  • [5] Seed germination and dormancy
    Bewley, JD
    [J]. PLANT CELL, 1997, 9 (07) : 1055 - 1066
  • [6] Bewley JD, 2013, SEEDS PHYSL DEV GERM
  • [7] PROPERTIES OF GLUTATHIONE-REDUCTASE FROM CHLOROPLASTS AND ROOTS OF PEA
    BIELAWSKI, W
    JOY, KW
    [J]. PHYTOCHEMISTRY, 1986, 25 (10) : 2261 - 2265
  • [8] Mathematically combined half-cell reduction potentials of low-molecular-weight thiols as markers of seed ageing
    Birtic, Simona
    Colville, Louise
    Pritchard, Hugh W.
    Pearce, Stephen R.
    Kranner, Ilse
    [J]. FREE RADICAL RESEARCH, 2011, 45 (09) : 1093 - 1102
  • [9] Spatial specificity of H2O2-generating oxalate oxidase gene expression during wheat embryo germination
    Caliskan, M
    Cuming, AC
    [J]. PLANT JOURNAL, 1998, 15 (02) : 165 - 171
  • [10] Intestinal redox biology and oxidative stress
    Circu, Magdalena L.
    Aw, Tak Yee
    [J]. SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2012, 23 (07) : 729 - 737