Muscle metabolic responses during 16 hours of intermittent heavy exercise

被引:4
|
作者
Green, H. J. [1 ]
Duhamel, T. A. [1 ]
Holloway, G. P. [1 ]
Moule, J. [1 ]
Ouyang, J. [1 ]
Ranney, D. [1 ]
Tupling, A. R. [1 ]
机构
[1] Univ Waterloo, Dept Kinesiol, Waterloo, ON N2L 3G1, Canada
关键词
metabolism; adaptation; repetitive activity; oxidative phosphorylation; mitochondrial sensitivity;
D O I
10.1139/Y07-039
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The alterations in muscle metabolism were investigated in response to repeated sessions of heavy intermittent exercise performed over 16 h. Tissue samples were extracted from the vastus lateralis muscle before (B) and after (A) 6 min of cycling at approximately 91% peak aerobic power at repetitions one (R1), two (R2), nine (R9), and sixteen (R16) in 13 untrained volunteers (peak aerobic power = 44.3 +/- 0.66 mL center dot kg(-1)center dot min(-1), mean +/- SE). Metabolite content (mu mol center dot(kg dry mass)(-1)) in homogenates at R1 indicated decreases (p < 0.05) in ATP (21.9 +/- 0.62 vs. 17.7 +/- 0.68) and phosphocreatine (80.3 +/- 2.0 vs. 8.56 +/- 1.5) and increases (p < 0.05) in inosine monophosphate (IMP, 0.077 +/- 0.12 vs. 3.63 +/- 0.85) and lactate (3.80 +/- 0.57 vs. 84.6 +/- 10.3). The content (mu mol center dot(kg dry mass)(-1)) of calculated free ADP ([ADP(f)], 86.4 +/- 5.5 vs. 1014 +/- 237) and free AMP ([AMP(f)], 0.32 +/- 0.03 vs. 78.4 +/- 31) also increased (p < 0.05). No differences were observed between R1 and R2. By R9 and continuing to R16, pronounced reductions (p < 0.05) at A were observed in IMP (72.2%), [ADP(f)] (58.7%), [AMP(f)] (85.5%), and lactate (41.3%). The 16-hour protocol resulted in an 89.7% depletion (p < 0.05) of muscle glycogen. Repetition-dependent increases were also observed in oxygen consumption during exercise. It is concluded that repetitive heavy exercise results in less of a disturbance in phosphorylation potential, possibly as a result of increased mitochondrial respiration during the rest-to-work non-steady-state transition.
引用
收藏
页码:634 / 645
页数:12
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