Homology modeling and docking study of β-ketoacyl acyl carrier protein synthase III from Enterococcus faecalis

被引:0
作者
Jeong, Ki-Woong
Lee, Jee-Young
Kim, Yangmee [1 ]
机构
[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea
[2] Konkuk Univ, Bio Mol Informat Ctr, Seoul 143701, South Korea
来源
BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2007年 / 28卷 / 08期
关键词
KAS III; Enterococcusfaecalis; fatty acid synthesis; antibiotics; flavonoids;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
beta-Ketoacyl acyl carrier protein synthase (KAS) III is a particularly attractive target in the type II fatty acid synthetic pathway, since it is central to the initiation of fatty acid synthesis. Enterococcus faecalis, a Gram-positive bacterium, is one of the major causes of hospital acquired infections. The rise of multidrug-resistant of most bacteria requires the development of new antibiotics, such as inhibition of the KAS III. In order to block the fatty acid synthesis by inhibition of KAS III, at first, three dimensional structure of Enterococcus-faecalis KAS III (efKAS HI) was determined by comparative homology modeling using MODELLER based on x-ray structure of Staphylococcus aureus KAS III (saKAS III) which is a gram-positive bacteria and is 36.1% identical in amino acid sequences with efKAS III. Since His-Asn-Cys catalytic triad is conserved in efKAS III and saKAS III, substrate specificity of efKAS III and saKAS III and the size of primer binding pocket of these two proteins are expected to be similar. Ligand docking study of efKAS III with naringenin and apigenin showed that naringenin docked more strongly with efKAS III than apigenin, resulting in the intensive hydrogen bond network between naringenin and efKAS III. Also, only naringenin showed antibacterial activity against E. faecalis at 256,mu g/mL. This study may give practical implications of flavonoids for antimicrobial effects against E. faecalis.
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页码:1335 / 1340
页数:6
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