Effect of madecassoside and catalpol in amelioration of insulin sensitivity in pancreatic (INS-1E) β-cell line

被引：9

作者：

Elhassan, Safa Abdelgadir Mohamed ^{[1
]}

Candasamy, Mayuren ^{[2
]}

Ching, Tan Swee ^{[1
]}

Heng, Yap Kah ^{[1
]}

Bhattamisra, Subrat Kumar ^{[2
]}

机构：

[1] Int Med Univ, Sch Postgrad Studies, Kuala Lumpur, Malaysia

[2] Int Med Univ, Sch Pharm, Dept Life Sci, Kuala Lumpur, Malaysia

来源：

NATURAL PRODUCT RESEARCH | 2021年 / 35卷 / 22期

关键词：

Madecassoside; Catalpol; Pancreatic INS-1E cells; RESISTANCE; SECRETION;

D O I：

10.1080/14786419.2019.1696794

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Currently, type 2 diabetes mellitus (T2D) has emerged as global burden disease. Herbal drugs with antidiabetic activities are attracting the attention. Madecassoside and catalpol are herbal compounds having strong antioxidant and glucose lowering activity. Madecassoside and catalpol were investigated for their effect on insulin sensitivity using pancreatic INS-1E cells. Cytotoxicity of these compounds was evaluated by MTT assay. Glucose-stimulated insulin secretion (GSIS) and expression of insulin signalling proteins were studied in presence of madecassoside and catalpol. Results revealed that madecassoside and catalpol enhanced the GSIS without cytotoxic effect. Madecassoside (30 mu M) and catalpol (40 mu M) increased the insulin secretion in response to high glucose (16.7 mM) stimulation. Subsequently, madecassoside and catalpol showed elevated expression of p-IRS-1, Akt, and p-Akt proteins. Madecassoside and catalpol after 24 h of incubation in pancreatic INS-1E cells with high glucose concentration (30 mM) ameliorated the insulin secretion.

引用

页码：4627 / 4631

页数：5

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