A selective fluorescence sensor for hypochlorite used for the detection of hypochlorite in zebrafish

被引：21

作者：

Kim, Pyeong An ^{[1
]}

Choe, Donghwan ^{[1
]}

So, Haeri ^{[1
]}

Park, Soyoung ^{[1
]}

Suh, Boeon ^{[1
]}

Jeong, Soomin ^{[2
]}

Kim, Ki-Tae ^{[2
]}

Kim, Cheal ^{[1
]}

Harrison, Roger G. ^{[3
]}

机构：

[1] Seoul Natl Univ Sci & Technol, Dept Fine Chem, Seoul 137743, South Korea

[2] Seoul Natl Univ Sci & Technol, Dept Environm Engn, Seoul 01187, South Korea

[3] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2021年 / 261卷

关键词：

Hypochlorite sensor; Fluorescent probe; Zebrafish; Imine oxidation; LIVE-CELL; PROBE; ACID; MEDIATOR; HCLO;

D O I：

10.1016/j.saa.2021.120059

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Hypochlorite is used as a water disinfectant and it is also produced by biological organisms. Its detection and quantification is important and could lead to its mechanism of reactivity in cells. We have synthesized a new fluorescence sensor for hypochlorite based on bithiophene and furan-carbohydrazide. The sensor shows increased fluorescence as a function of hypochlorite and is selective for hypochlorite. Fluorescence enhancement due to hypochlorite is observed when the sensor is used in aqueous solutions at neutral pH values. Using the sensor, the detection limit for hypochlorite is 4.2 mM, making the sensor practical to determine hypochlorite in water. Applying the sensor to aide in the detection of hypochlorite in zebrafish, showed localization of ClO-/HClO in the air bladders and eyes of zebrafish. (C) 2021 Elsevier B.V. All rights reserved.

引用

页数：6

共 31 条

[1] An oxidative cyclization reaction based fluorescent "Turn-On" probe for highly selective and rapid detection of hypochlorous acid [J].

Chen, Bo ;

Fu, Hongyu ;

Lv, Yaohong ;

Li, Xin ;

Han, Yifeng .

TETRAHEDRON LETTERS, 2018, 59 (12) :1116-1120

[2] A Triphenyl Amine-Based Solvatofluorochromic Dye for the Selective and Ratiometric Sensing of OCl- in Human Blood Cells [J].

Goswami, Shyamaprosad ;

Aich, Krishnendu ;

Das, Sangita ;

Pakhira, Bholanath ;

Ghoshal, Kakali ;

Quah, Ching Kheng ;

Bhattacharyya, Maitree ;

Fun, Hoong-Kun ;

Sarkar, Sabyasachi .

CHEMISTRY-AN ASIAN JOURNAL, 2015, 10 (03) :694-700

[3] A new turn-on fluorescent probe towards hypochlorite in living cells [J].

Guo, Jing ;

Zhang, Zhixiang ;

Kuai, Ziyu ;

Wang, Rui ;

Yang, Qingbiao ;

Shan, Yaming ;

Li, Yaoxian .

ANALYTICAL METHODS, 2017, 9 (05) :864-870

[4] Myeloperoxidase-dependent generation of hypochlorite-modified proteins in human placental tissues during normal pregnancy [J].

Hammer, A ;

Desoye, G ;

Dohr, G ;

Sattler, WG ;

Malle, E .

LABORATORY INVESTIGATION, 2001, 81 (04) :543-554

[5] A ratiometric fluorescent probe for in situ quantification of basal mitochondrial hypochlorite in cancer cells [J].

Hou, Ji-Ting ;

Li, Kun ;

Yang, Jin ;

Yu, Kang-Kang ;

Liao, Ye-Xin ;

Ran, Yu-Zhao ;

Liu, Yan-Hong ;

Zhou, Xue-Dong ;

Yu, Xiao-Qi .

CHEMICAL COMMUNICATIONS, 2015, 51 (31) :6781-6784

[6] HKOCl-3: a fluorescent hypochlorous acid probe for live-cell and in vivo imaging and quantitative application in flow cytometry and a 96-well microplate assay [J].

Hu, Jun Jacob ;

Wong, Nai-Kei ;

Lu, Ming-Yang ;

Chen, Xingmiao ;

Ye, Sen ;

Zhao, Angela Qian ;

Gao, Peng ;

Kao, Richard Yi-Tsun ;

Shen, Jiangang ;

Yang, Dan .

CHEMICAL SCIENCE, 2016, 7 (03) :2094-2099

[7] A simple hydrazine-based probe bearing anthracene moiety for the highly selective detection of hypochlorite [J].

Hwang, Suh Mi ;

Kim, Ahran ;

Kim, Cheal .

INORGANIC CHEMISTRY COMMUNICATIONS, 2019, 101 :1-5

[8] A fast response fluorescence probe specific for hypochlorous acid detection and its applications in bioimaging [J].

Jia, Hongmin ;

Xia, Shuhe ;

Feng, Huan ;

Meng, Qingtao ;

Duan, Chengchen ;

Zhang, Zhiqiang ;

Zhang, Run .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2018, 16 (12) :2074-2082

[9] Novel diaminomaleonitrile-based fluorescent probe for ratiometric detection and bioimaging of hypochlorite [J].

Jiang, Yuliang ;

Wu, Shishan ;

Jin, Can ;

Wang, Bingxiang ;

Shen, Jian .

SENSORS AND ACTUATORS B-CHEMICAL, 2018, 265 :365-370

[10] Fluorescence probe for hypochlorous acid in water and its applications for highly lysosome-targetable live cell imaging [J].

Jiao, Xiaojie ;

Liu, Chang ;

Wang, Qing ;

Huang, Kun ;

He, Song ;

Zhao, Liancheng ;

Zeng, Xianshun .

ANALYTICA CHIMICA ACTA, 2017, 969 :49-56

← 1 2 3 4 →