Impact and Possible Mechanism(s) of Adipose Tissue-Derived Mesenchymal Stem Cells on T-Cell Proliferation in Patients With Rheumatic Disease

被引:10
作者
Kuca-Warnawin, Ewa [1 ]
Olesinska, Marzena [2 ]
Kontny, Ewa [1 ]
机构
[1] Natl Inst Geriatr Rheumatol & Rehabil, Dept Pathophysiol & Immunol, Warsaw, Poland
[2] Natl Inst Geriatr Rheumatol & Rehabil, Clin Connect Tissue Dis, Warsaw, Poland
关键词
systemic lupus erythematosus; systemic sclerosis; adipose-derived mesenchymal stem cells; T-cell proliferation; soluble mediators; SYSTEMIC-LUPUS-ERYTHEMATOSUS; STROMAL CELLS; LYMPHOCYTE-PROLIFERATION; BONE-MARROW; CLASSIFICATION CRITERIA; MURINE MODEL; B-CELLS; SCLEROSIS; PATHOGENESIS; CONTACT;
D O I
10.3389/fphys.2021.749481
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Objectives: Systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) are chronic wasting, incurable rheumatic diseases of autoimmune background, in which T cells play a critical pathogenic role. Autologous adipose tissue-derived mesenchymal stem cells (ASCs) may represent an alternative therapeutic option for SLE and SSc patients, but the biology of these cells is poorly understood.Methods: Herein, we evaluated the anti-proliferative impact of ASCs of healthy donors (HD/ASCs, 5 reference cell lines), SLE patients (n = 20), and SSc patients (n = 20) on T lymphocytes. To assess the direct and indirect pathway of ASCs action, peripheral blood mononuclear cells (PBMCs) and purified CD4+ T cells of HD were activated and co-cultured in cell-to-cell contact (C-C) and transwell (T-W) conditions with untreated or cytokine (TNF + IFN, TI)-licensed ASCs, then analyzed by flow cytometry to rate the proliferation response of CD8(+) and/or CD4(+) T cells. The concentrations of kynurenines, prostaglandin E2 (PGE(2)), interleukin 10 (IL-10), and transforming growth factor beta (TGF beta) were measured from culture supernatants. Specific inhibitors of these factors (1-MT, indomethacin, and cytokine-neutralizing antibody) were used to assess their contribution to anti-proliferative ASCs action.Results: All tested ASCs significantly decreased the number of proliferating CD4(+) and CD8(+) T cells, the number of division/proliferating cell (PI), and fold expansion (RI), and similarly upregulated kynurenines and PGE(2), but not cytokine levels, in the co-cultures with both types of target cells. However, TI-treated SLE/ASCs and SSc/ASCs exerted a slightly weaker inhibitory effect on CD4(+) T-cell replication than their respective HD/ASCs. All ASCs acted mainly via soluble factors. Their anti-proliferative effect was stronger, and kynurenine levels were higher in the T-W condition than the C-C condition. Blocking experiments indicated an involvement of kynurenine pathway in inhibiting the number of proliferating cells, PI, and RI values as well as PGE(2) role in decreasing the number of proliferating cells. TGF beta did not contribute to ASCs anti-proliferative capabilities, while IL-10 seems to be involved in such activity of only SLE/ASCs.Conclusion: The results indicate that SLE/ASCs and SSc/ASCs retain their capability to restrain the expansion of allogeneic CD4(+) and CD8(+) T cells and act by similar mechanisms as ASCs of healthy donors and thus may have therapeutic value.
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页数:16
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