Design of an Integrated Microfluidic Paper-Based Chip and Inspection Machine for the Detection of Mercury in Food with Silver Nanoparticles

被引：11

作者：

Fu, Lung-Ming ^{[1
,2
]}

Shih, Ming-Kuei ^{[3
]}

Hsieh, Chang-Wei ^{[4
,5
]}

Ju, Wei-Jhong ^{[1
]}

Tain, You-Lin ^{[6
,7
,8
]}

Cheng, Kuan-Chen ^{[9
,10
,11
,12
]}

Hsu, Jia-Hong ^{[13
]}

Chen, Yu-Wei ^{[14
]}

Hou, Chih-Yao ^{[1
,2
]}

机构：

[1] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701401, Taiwan

[2] Natl Pingtung Univ Sci & Technol, Grad Inst Mat Engn, Pingtung 912301, Taiwan

[3] Natl Kaohsiung Univ Hospitality & Tourism, Grad Inst Food Culture & Innovat, Kaohsiung 812301, Taiwan

[4] Natl Chung Hsing Univ, Dept Food Sci & Biotechnol, Taichung 402202, Taiwan

[5] China Med Univ Hosp, Dept Med Res, Taichung 404328, Taiwan

[6] Kaohsiung Chang Gung Mem Hosp, Dept Pediat, Kaohsiung 833401, Taiwan

[7] Chang Gung Univ, Coll Med, Kaohsiung 833401, Taiwan

[8] Kaohsiung Chang Gung Mem Hosp, Inst Translat Res Biomed, Kaohsiung 833401, Taiwan

[9] Natl Taiwan Univ, Coll Bioresources & Agr, Inst Food Sci & Technol, Taipei 106216, Taiwan

[10] Natl Taiwan Univ, Inst Biotechnol, Coll Bioresources & Agr, Taipei 106216, Taiwan

[11] China Med Univ, China Med Univ Hosp, Dept Med Res, 91 Hsueh Shih Rd, Taichung 404328, Taiwan

[12] Asia Univ, Dept Optometry, Taichung 413305, Taiwan

[13] Natl Kaohsiung Univ Sci & Technol, Dept Seafood Sci, Kaohsiung 811213, Taiwan

[14] Chang Gung Univ, Dept Med, Linkow 333423, Taiwan

来源：

BIOSENSORS-BASEL | 2021年 / 11卷 / 12期

关键词：

microfluidic paper-based analytical device (mu PAD); silver nanoparticles (AgNPs); mercury (Hg); RGB; ANALYTICAL DEVICES; COLORIMETRIC DETECTION; RAPID-DETERMINATION; REACTIVE PHOSPHATE; CYTO-DEVICE; METAL; GOLD; REDUCTION; PLATFORM; ARRAYS;

D O I：

10.3390/bios11120491

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

For most of the fast screening test papers for detecting Hg2+, the obtained results are qualitative. This study developed an operation for the mu PAD and combined it with the chemical colorimetric method. Silver nanoparticle (AgNP) colloids were adopted as the reactive color reagent to combine and react with the Hg standards on the paper-based chip. Then, the RGB values for the color change were used to establish the standard curve (R-2 > 0.99). Subsequently, this detection system was employed for the detection tests of actual samples, and the detected RGB values of the samples were substituted back to the formula to calculate the Hg2+ contents in the food. In this study, the Hg2+ content and recovery rate in commercially available packaged water and edible salts were measured. The research results indicate that a swift, economical, and simple detection method for Hg2+ content in food has been successfully developed.

引用

页数：15

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