Improved method to track chlorophyll degradation

被引:60
作者
Gauthier-Jaques, A [1 ]
Bortlik, K [1 ]
Hau, J [1 ]
Fay, LB [1 ]
机构
[1] Nestec Ltd, Nestle Res Ctr, CH-1000 Lausanne 26, Switzerland
关键词
chlorophyll; food; beans; spinach; degradation; vegetables; analytical; LC; MS; MS/MS; fragmentation; spectra; processing;
D O I
10.1021/jf000384c
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
An analytical method capable of identifying > 30 chlorophyll-related compounds in plant extracts has been developed. The method employs liquid chromatography coupled to UV-vis, MS, and MS/ MS detection. It can be applied without modification to analyze natural chlorophyll degradation products and other metalloporphyrines. It was successfully applied to identify chlorophyll derivatives found in rehydrated spinach powder and conventionally canned and Veri-Green-processed beans. In the Veri-Green-processed beans several degradation products were identified that are zinc-containing analogues to the chlorophyll derivatives found in vegetables after conventional canning. They have been characterized by liquid chromatography and mass spectrometry.
引用
收藏
页码:1117 / 1122
页数:6
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