EFFECTIVE EXTRACTION OF THE YEASTDERIVED LACTASE BY HIGH-VOLTAGE PULSED ELECTRIC FIELD

被引：0

作者：

Abe, Tsubasa ^{[1
]}

Yamazaki, Satoshi ^{[1
]}

Minamitani, Yasushi ^{[1
]}

Sugiura, Toshiyuki ^{[2
]}

机构：

[1] Yamagata Univ, Grad Sch Sci & Engn, 4-3-16 Jonan, Yonezawa, Yamagata 9928510, Japan

[2] Amano Enzyme Inc, Kitanagoya, Aichi 4818533, Japan

来源：

2015 IEEE PULSED POWER CONFERENCE (PPC) | 2015年

关键词：

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Industrial uses of an enzyme in the fields of medicine and foods are increasing. Lactase is one of those enzymes. Since the lactase derived from yeast exists in the cell body, it is obtained from inside of the cell by destroying the cell membrane by ultrasonic disintegration conventionally. Recently, the new collection method of enzyme from the inside of cells using the high voltage pulse electric field (PEF) has been studied. Also this study focuses on extracting the lactase from pores forming on the cell membrane effectively by applying PEF to Kluyveromyces lactis. As a result, intracellular lactase has been able to be extracted to the culture medium by applying PEF to K. lactis.

引用

页数：4

共 50 条

[21] Cascaded boost converter-based high-voltage pulse generator for pulsed electric field applications
Krishnavenio, S.
Rajini, V.
ARCHIVES OF ELECTRICAL ENGINEERING, 2021, 70 (03) : 631 - 641
[22] STIMULATED-EMISSION IN GALLIUM-ARSENIDE EXCITED BY HIGH-VOLTAGE PULSED ELECTRIC-FIELD
GRIBKOVSKY, VP
IVANOV, VA
PARASHCHUK, VV
RYABTSEV, GI
YABLONSKY, GP
KVANTOVAYA ELEKTRONIKA, 1978, 5 (09): : 2044 - 2046
[23] Compact High-Voltage Pulse Generator for Pulsed Electric Field Applications: Lab-Scale Development
Kasri, N. F.
Piah, M. A. M.
Adzis, Z.
JOURNAL OF ELECTRICAL AND COMPUTER ENGINEERING, 2020, 2020
[24] Development of a High-Voltage Pulsed Electric Field Sterilization Power Supply Using a New Topology Circuit
Zhu, Bo
Su, He
Fang, Zhihan
Wu, Guoyan
Wei, Xinlao
ENERGIES, 2023, 16 (06)
[25] Review of the Dynamics of Coalescence and Demulsification by High-Voltage Pulsed Electric Fields
Peng, Ye
Liu, Tao
Gong, Haifeng
Zhang, Xianming
INTERNATIONAL JOURNAL OF CHEMICAL ENGINEERING, 2016, 2016
[26] Releasing profiles of gene products from recombinant Escherichia coli in a high-voltage pulsed electric field
Ohshima, T
Hama, Y
Sato, M
BIOCHEMICAL ENGINEERING JOURNAL, 2000, 5 (02) : 149 - 155
[27] HIGH-VOLTAGE PULSED ELECTRIC-FIELD INACTIVATION OF BACILLUS-SUBTILIS AND LACTOBACILLUS-DELBRUECKII
POTHAKAMURY, UR
MONSALVEGONZALEZ, A
BARBOSACANOVAS, GV
SWANSON, BG
REVISTA ESPANOLA DE CIENCIA Y TECNOLOGIA DE ALIMENTOS, 1995, 35 (01): : 101 - 107
[28] Development of controller for high-voltage pulsed electric field generator device based on field-programmable gate array
Zhang, Ruobing
Zhang, Xing
Liu, Huaiyu
Chen, Zhiyuan
Gaodianya Jishu/High Voltage Engineering, 2015, 41 (12): : 4021 - 4026
[29] CONTROLLED HIGH-VOLTAGE DISCHARGER WITH ELECTRIC-FIELD DISTORTION
BARANOV, VV
BELOGLAZOV, AA
DUDIN, AY
PERLOV, SG
ROMANOV, AV
USTINOVSKII, NN
KHOLIN, IV
INSTRUMENTS AND EXPERIMENTAL TECHNIQUES, 1989, 32 (02) : 392 - 395
[30] EFFICIENT COMPUTATION OF ELECTRIC-FIELD IN HIGH-VOLTAGE EQUIPMENT
QIN, BL
CHEN, GZ
QIAO, Q
SHENG, JN
IEEE TRANSACTIONS ON MAGNETICS, 1990, 26 (02) : 387 - 390

← 1 2 3 4 5 →