Production and the characterization of monoclonal antibody against CD43, K06

被引：6

作者：

Park, WS

Chae, JS

Jung, KC

Choi, WJ

Kook, MC

Bae, Y ^{[1
]}

机构：

[1] Kangwon Natl Univ, Coll Med, Dept Pathol, Chunchon 200701, Kangwon Do, South Korea

[2] Kangwon Natl Univ Hosp, Clin Res Inst, Chunchon, South Korea

[3] Hallym Univ, Coll Med, Dept Pathol, Chunchon, South Korea

[4] Kangwon Natl Univ, Coll Med, Dept Surg, Chunchon, South Korea

[5] Seoul Natl Univ, Coll Med, Dept Pathol, Seoul 151, South Korea

来源：

TISSUE ANTIGENS | 2004年 / 63卷 / 01期

关键词：

anti-CD43; antibody; development; expression pattern; glycosylation; thymocytes;

D O I：

10.1111/j.1399-0039.2004.00151.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A monoclonal antibody against human CD43 has been developed and designated as K06. Its reactivity in the lymphoid organs was different from that of known anti-CD43 monoclonal antibodies suggesting that this may recognize a novel epitope of human CD43 molecule. The CD43 epitope detected by anti-K06 monoclonal antibody was highly expressed in cortical thymocytes, platelets, and myeloid cells of normal peripheral blood, and its reactivity was comparable to that of known anti-CD43 monoclonal antibodies. However, the density of this epitope was lower in the medullary thymocytes. Biochemical studies indicated that anti-K06 monoclonal antibody could recognize glycosylated moiety of CD43 antigen. The expression profile of anti-K06 monoclonal antibody in several cell lines was somewhat different from that of known anti-CD43 antibodies. In addition, CD43 ligation through the K06 epitope appeared to induce apoptosis in human leukemic cell line, Molt-4. We therefore assume that K06 epitope of human CD43 might have some role in T-cell development.

引用

页码：46 / 53

页数：8

共 23 条

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