Hybridization chain reaction directed DNA superstructures assembly for biosensing applications

被引：87

作者：

Yang, Dawei ^{[1
]}

Tang, Yuguo ^{[1
]}

Miao, Peng ^{[1
]}

机构：

[1] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, CAS Key Lab Biomed Diagnost, Suzhou 215163, Peoples R China

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2017年 / 94卷

基金：

中国国家自然科学基金;

关键词：

Hybridization chain reaction; Isothermal amplification; Nanotechnology; Biosensor; Ultrahigh sensitivity; ROLLING-CIRCLE AMPLIFICATION; STRAND-DISPLACEMENT POLYMERIZATION; MEDIATED ISOTHERMAL AMPLIFICATION; HIGHLY SENSITIVE DETECTION; DUAL SIGNAL AMPLIFICATION; ENZYME-FREE; GOLD NANOPARTICLES; LABEL-FREE; ELECTROCHEMICAL DETECTION; NUCLEIC-ACIDS;

D O I：

10.1016/j.trac.2017.06.011

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hybridization chain reaction (HCR) is a simple isothermal enzyme-free process with the advantages of high simplicity and versatility. Generally, initiator single-stranded DNA is amplified to form long double stranded DNA based on a chain reaction, during which recognition and hybridization events between a pair of complementary DNA sequences occur in succession. Repeating units of HCR product can be further utilized as templates for the assembly of various nanospecies, generating complicated DNA superstructures. Therefore, HCR is regarded as an attractive technique for DNA nanotechnology, biosensing, bioimaging and biomedicine. Particularly, HCR has been utilized to develop promising biosensors for the detection of various small molecules, nucleic acids, proteins and cells. In this review, we provide a comprehensive overview of HCR directed DNA superstructures assembly for biosensing applications. We hope this review will offer readers general concept of HCR and stimulate interest, new ideas, and discoveries in the fascinating field. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：1 / 13

页数：13

共 88 条

[1] Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine [J].