DNAzyme-powered nucleic acid release from solid supports

被引：4

作者：

Cao, Ting ^{[1
,2
,3
,4
]}

Wang, Yongcheng ^{[1
,2
,3
,5
]}

Tao, Ye ^{[1
,2
]}

Zhang, Lexiang ^{[1
,2
]}

Zhou, Ying-Lin ^{[4
]}

Zhang, Xin-Xiang ^{[4
]}

Heyman, John A. ^{[1
,2
]}

Weitz, David A. ^{[1
,2
,3
]}

机构：

[1] Harvard Univ, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[2] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

[3] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[4] Peking Univ, Coll Chem & Mol Engn, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci BNLMS, Beijing 100871, Peoples R China

[5] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 04期

关键词：

RNA; DNA; ENZYME;

D O I：

10.1039/c9cc07790a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, we demonstrate use of a Mg2+-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

引用

页码：647 / 650

页数：4

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