HPLC quantitation of kaurane diterpenes in Xylopia species

被引：19

作者：

de Melo, AC ^{[1
]}

Cota, BB ^{[1
]}

de Oliveira, AB ^{[1
]}

Braga, FC ^{[1
]}

机构：

[1] UFMG, Fac Farm, BR-30180112 Belo Horizonte, MG, Brazil

来源：

FITOTERAPIA | 2001年 / 72卷 / 01期

关键词：

Xylopia species; kaurenoic acid; xylopic acid; 16-alpha-hydroxykauranoic acid; HPLC quantitation;

D O I：

10.1016/S0367-326X(00)00251-3

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Xylopia frutescens is a tree native to the Brazilian Amazon whose seeds are rich in kaurenoic acid, a diterpene that showed in vitro activity against Trypanosoma cruzi. Aiming to find out alternative sources for kaurenoic acid, the content of some kaurane diterpenes was evaluated in X. aromatica and X. brasiliensis, species occurring in the Cerrado area of Minas Gerais, and also in X. fructescens. A reversed phase HPLC isocratic method was developed and validated to perform the assays. Kaurenoic acid was found to be the most abundant diterpene within the analyzed species, with a 3.16 +/- 0.97% content in the seeds of X. frutescens, which also presented the highest amount of xylopic acid (1.09 +/- 0.33%). The highest concentration of 16-alpha -hydroxykauranoic acid (1.96 +/- 1.58%) was found in the stems of X. aromatica. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：40 / 45

页数：6

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