Efficient biodegradation of phenanthrene by a novel strain Massilia sp WF1 isolated from a PAH-contaminated soil

被引：79

作者：

Wang, Haizhen ^{[1
]}

Lou, Jun ^{[1
]}

Gu, Haiping ^{[1
]}

Luo, Xiaoyan ^{[1
]}

Yang, Li ^{[1
]}

Wu, Laosheng ^{[1
,2
]}

Liu, Yong ^{[1
,3
]}

Wu, Jianjun ^{[1
]}

Xu, Jianming ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Soil & Water Resources & Environm Sci, Coll Environm & Resource Sci, Zhejiang Prov Key Lab Agr Resources & Environm, Hangzhou 310058, Zhejiang, Peoples R China

[2] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA

[3] Guangdong Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Agroenvironm Integrated Control, Guangzhou 510650, Guangdong, Peoples R China

来源：

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 2016年 / 23卷 / 13期

基金：

中国国家自然科学基金;

关键词：

Massilia sp; WF1; Phenanthrene; Biodegradation; Metabolic pathway; POLYCYCLIC AROMATIC-HYDROCARBONS; SP-NOV; DEGRADATION; ACID; CONSORTIUM;

D O I：

10.1007/s11356-016-6515-6

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A novel phenanthrene (PHE)-degrading strain Massilia sp. WF1, isolated from PAH-contaminated soil, was capable of degrading PHE by using it as the sole carbon source and energy in a range of pH (5.0-8.0), temperatures (20-35 A degrees C), and PHE concentrations (25-400 mg L-1). Massilia sp. WF1 exhibited highly effective PHE-degrading ability that completely degraded 100 mg L-1 of PHE over 2 days at optimal conditions (pH 6.0, 28 A degrees C). The kinetics of PHE biodegradation by Massilia sp. WF1 was well represented by the Gompertz model. Results indicated that PHE biodegradation was inhibited by the supplied lactic acid but was promoted by the supplied carbon sources of glucose, citric acid, and succinic acid. Salicylic acid (SALA) and phthalic acid (PHTA) were not utilized by Massilia sp. WF1 and had no obvious effect on PHE biodegradation. Only two metabolites, 1-hydroxy-2-naphthoic acid (1H2N) and PHTA, were identified in PHE biodegradation process. Quantitatively, nearly 27.7 % of PHE was converted to 1H2N and 30.3 % of 1H2N was further metabolized to PHTA. However, the PHTA pathway was broken and the SALA pathway was ruled out in PHE biodegradation process by Massilia sp. WF1.

引用

页码：13378 / 13388

页数：11

共 37 条

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