Bacillus halochares sp nov., a halophilic bacterium isolated from a solar saltern

被引:17
作者
Pappa, A. [1 ]
Sanchez-Porro, C. [2 ]
Lazoura, P. [1 ]
Kallimanis, A. [1 ]
Perisynakis, A. [1 ]
Ventosa, A. [2 ]
Drainas, C. [1 ]
Koukkou, A. I. [1 ]
机构
[1] Univ Ioannina, Dept Chem, Sector Organ Chem & Biochem, GR-45110 Ioannina, Greece
[2] Univ Seville, Fac Pharm, Dept Microbiol & Parasitol, E-41012 Seville, Spain
基金
美国国家科学基金会;
关键词
GEN; NOV; DIVERSITY; IDENTIFICATION; ENVIRONMENT; PROPOSAL; MEMBER; LAKE;
D O I
10.1099/ijs.0.014233-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel halophilic bacterium, designated strain MSS4(T), was isolated from the solar salterns of Mesolongi, Greece. The micro-organism, a motile, Gram-stain-positive, aerobic rod, proliferated at salinities of 1.0-4.0 M NaCl, with optimal growth at 2.5 M NaCl. Endospores were not observed. Strain MSS4(T) showed optimal growth at 37 degrees C and pH 8.0. The G+C content of its DNA was 47.2 mol%. The polar lipid pattern of strain MSS4(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidic acid and phosphatidylethanolamine. It possessed anteiso-C-15:0, C-18:0, C-16:0 and anteiso-C-17:0 as the major fatty acids (altogether representing 84.7% of the total). The predominant isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. 16S rRNA gene sequence analysis showed that the new isolate has 96.1% similarity to Bacillus qingdaonensis CM1(T) and Bacillus aidingensis 17-5(T), 95.5% to Bacillus salarius BH169(T) and lower similarity to other Bacillus species. These results justify the assignment of strain MSS4(T) to a novel species within the genus Bacillus, for which the name Bacillus halochares sp. nov. is proposed. The type strain is MSS4(T) (=LMG 24571(T) =DSM 21373(T)).
引用
收藏
页码:1432 / 1436
页数:5
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