Mfsd2a Promotes the Proliferation, Migration, Differentiation and Adipogenesis of Chicken Intramuscular Preadipocytes

被引：3

作者：

Lin, Z. Z. ^{[1
]}

Li, Z. Q. ^{[1
]}

Li, J. J. ^{[1
]}

Yu, C. L. ^{[2
]}

Yang, C. W. ^{[2
]}

Ran, J. S. ^{[1
]}

Yin, L. Q. ^{[1
]}

Zhang, D. H. ^{[1
]}

Zhang, G. F. ^{[1
]}

Liu, Y. P. ^{[1
]}

机构：

[1] Sichuan Agr Univ, Farm Anim Genet Resources Explorat & Innovat Key, 211 Huiming Rd, Chengdu 611130, Sichuan, Peoples R China

[2] Sichuan Anim Sci Acad, Anim Breeding & Genet Key Lab Sichuan Prov, 7 Niusha Rd, Chengdu 610066, Sichuan, Peoples R China

来源：

BRAZILIAN JOURNAL OF POULTRY SCIENCE | 2022年 / 24卷 / 01期

关键词：

Chicken; intramuscular fat; Mfsd2a; PPAR gamma; signaling pathway; preadipocytes; TRANSPORTER; RECEPTOR; PATHWAY; MUSCLE; TISSUE;

D O I：

10.1590/1806-9061-2021-1547

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Intramuscular fat (IMF) content is a crucial parameter for estimating meat quality. Growing evidence indicates that gene regulation plays an important role in IMF deposition. This study aimed to determine the function of Mfsd2a in chicken intramuscular preadipocytes. In the present study, high Mfsd2a mRNA levels were observed in the liver and adipose tissues of broilers. Subsequently, we synthesized small interfering RNAs to silence the expression of Mfsd2a in chicken intramuscular preadipocytes. The following results suggested that CDK2, PCNA, CCND1, CCND2 and MKI67 were inhibited, with CCK-8 and EdU assays revealing that cell proliferation was inhibited. Scratch test showed that cell migration ratios were declined. We also found that Mfsd2a silencing decreased the mRNA levels of PPAR., RXRG and their target genes. The similar results were found in some key genes that contribute to lipid synthesis, including C/EBP alpha, C/EBP beta, FABP4, FASN, ACACA and ACSL1. Finally, Oil red O staining showed that IMF accumulation was blocked after Mfsd2a silencing. In conclusion, our results implied that Mfsd2a promotes the proliferation and migration of chicken intramuscular preadipocytes, as well as the differentiation and adipogenesis through PPAR. signaling pathway, which may provide a potential target to improve chicken meat quality.

引用

页数：8

共 40 条

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