Influence of Diet, Sex, and Viral Infections on the Gut Microbiota Composition of Spodoptera exigua Caterpillars

被引：44
作者：
Martinez-Solis, Maria [1 ]
Carmen Collado, Maria [2 ]
Herrero, Salvador [1 ]
机构：
[1] Univ Valencia, Dept Genet, Estruct Recerca Interdisciplinar Biotecnol & Biom, Valencia, Spain
[2] Consejo Super Invest Cient IATA CSIC, Inst Agroquim & Tecnol Alimentos, Valencia, Spain
来源：
FRONTIERS IN MICROBIOLOGY | 2020年 / 11卷
基金：
欧盟地平线“2020”;
关键词：
lepidoptera; microbiota; Spodoptera exigua; 16S rRNA; viral infection; BACILLUS-THURINGIENSIS; INSECTICIDE RESISTANCE; LEPIDOPTERA-NOCTUIDAE; BACTERIAL COMMUNITY; MIDGUT MICROBIOTA; FIELD POPULATIONS; LARVAL MIDGUT; EUREMA-HECABE; Z-CHROMOSOME; BOMBYX-MORI;
D O I：
10.3389/fmicb.2020.00753
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The gut microbiota plays essential roles in processes related with metabolism, physiology, and immunity in all organisms, including insects. In the present work, we performed a broad analysis of the Spodoptera exigua gut microbiota, a major agricultural pest. We analyzed the influence of multiple parameters such as diet, geographic location, sex, or viral infections on S. exigua caterpillar gut microbiota composition. Our study revealed a high variability in bacterial composition among individuals, and a major influence of environmental bacteria (including those acquired through diet) on the gut microbiota composition, supporting previous studies that claim resident microbiota are lacking in caterpillars. Previous studies with laboratory-reared insects showed that changes in caterpillar gut bacterial composition affect the insecticidal properties of entomopathogenic viruses and bacteria. Our study revealed different microbiota composition in field insects carrying a natural viral infection with Spodoptera exigua nucleopolyhedrovirus (SeMNPV) and/or Spodoptera exigua iflavirus 1 (SeIV1). Few taxa can be specifically associated with the infection, suggesting microbiota influence the infective process of these natural pathogens, and providing new strategies for insect pest management.
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页数：11
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