Influence of Diet, Sex, and Viral Infections on the Gut Microbiota Composition of Spodoptera exigua Caterpillars

被引:41
作者
Martinez-Solis, Maria [1 ]
Carmen Collado, Maria [2 ]
Herrero, Salvador [1 ]
机构
[1] Univ Valencia, Dept Genet, Estruct Recerca Interdisciplinar Biotecnol & Biom, Valencia, Spain
[2] Consejo Super Invest Cient IATA CSIC, Inst Agroquim & Tecnol Alimentos, Valencia, Spain
来源
FRONTIERS IN MICROBIOLOGY | 2020年 / 11卷
基金
欧盟地平线“2020”;
关键词
lepidoptera; microbiota; Spodoptera exigua; 16S rRNA; viral infection; BACILLUS-THURINGIENSIS; INSECTICIDE RESISTANCE; LEPIDOPTERA-NOCTUIDAE; BACTERIAL COMMUNITY; MIDGUT MICROBIOTA; FIELD POPULATIONS; LARVAL MIDGUT; EUREMA-HECABE; Z-CHROMOSOME; BOMBYX-MORI;
D O I
10.3389/fmicb.2020.00753
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The gut microbiota plays essential roles in processes related with metabolism, physiology, and immunity in all organisms, including insects. In the present work, we performed a broad analysis of the Spodoptera exigua gut microbiota, a major agricultural pest. We analyzed the influence of multiple parameters such as diet, geographic location, sex, or viral infections on S. exigua caterpillar gut microbiota composition. Our study revealed a high variability in bacterial composition among individuals, and a major influence of environmental bacteria (including those acquired through diet) on the gut microbiota composition, supporting previous studies that claim resident microbiota are lacking in caterpillars. Previous studies with laboratory-reared insects showed that changes in caterpillar gut bacterial composition affect the insecticidal properties of entomopathogenic viruses and bacteria. Our study revealed different microbiota composition in field insects carrying a natural viral infection with Spodoptera exigua nucleopolyhedrovirus (SeMNPV) and/or Spodoptera exigua iflavirus 1 (SeIV1). Few taxa can be specifically associated with the infection, suggesting microbiota influence the infective process of these natural pathogens, and providing new strategies for insect pest management.
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页数:11
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共 56 条
[1]   Fall Armyworm-Associated Gut Bacteria Modulate Plant Defense Responses [J].
Acevedo, Flor E. ;
Peiffer, Michelle ;
Tan, Ching-Wen ;
Stanley, Bruce A. ;
Stanley, Anne ;
Wang, Jie ;
Jones, Asher G. ;
Hoover, Kelli ;
Rosa, Cristina ;
Luthe, Dawn ;
Felton, Gary .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2017, 30 (02) :127-137
[2]   Resistance of beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) to endosulfan, organophosphorus and pyrethroid insecticides in Pakistan [J].
Ahmad, Mushtaq ;
Arif, M. Iqbal .
CROP PROTECTION, 2010, 29 (12) :1428-1433
[3]   Wolbachia in butterflies and moths: geographic structure in infection frequency [J].
Ahmed, Muhammad Z. ;
Araujo-Jnr, Eli V. ;
Welch, John J. ;
Kawahara, Akito Y. .
FRONTIERS IN ZOOLOGY, 2015, 12
[4]  
Aronesty E., 2011, Expr. Anal. Durham
[5]   Molecular sexing of Lepidoptera [J].
Belousova, Irina ;
Ershov, Nikita ;
Pavlushin, Sergey ;
Ilinsky, Yury ;
Martemyanov, Vyacheslav .
JOURNAL OF INSECT PHYSIOLOGY, 2019, 114 :53-56
[6]   Cascading effects on bacterial communities: cattle grazing causes a shift in the microbiome of a herbivorous caterpillar [J].
Berman, Tali S. ;
Laviad-Shitrit, Sivan ;
Lalzar, Maya ;
Halpern, Malka ;
Inbar, Moshe .
ISME JOURNAL, 2018, 12 (08) :1952-1963
[7]   FIELD MONITORING FOR INSECTICIDE RESISTANCE IN BEET ARMYWORM (LEPIDOPTERA, NOCTUIDAE) [J].
BREWER, MJ ;
TRUMBLE, JT .
JOURNAL OF ECONOMIC ENTOMOLOGY, 1989, 82 (06) :1520-1526
[8]   Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods [J].
Broderick, NA ;
Raffa, KF ;
Goodman, RM ;
Handelsman, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (01) :293-300
[9]   Midgut microbiota and host immunocompetence underlie Bacillus thuringiensis killing mechanism [J].
Caccia, Silvia ;
Di Lelio, Ilaria ;
La Storia, Antonietta ;
Marinelli, Adriana ;
Varricchio, Paola ;
Franzetti, Eleonora ;
Banyuls, Nuria ;
Tettamanti, Gianluca ;
Casartelli, Morena ;
Giordana, Barbara ;
Ferre, Juan ;
Gigliotti, Silvia ;
Ercolini, Danilo ;
Pennacchio, Francesco .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (34) :9486-9491
[10]   QIIME allows analysis of high-throughput community sequencing data [J].
Caporaso, J. Gregory ;
Kuczynski, Justin ;
Stombaugh, Jesse ;
Bittinger, Kyle ;
Bushman, Frederic D. ;
Costello, Elizabeth K. ;
Fierer, Noah ;
Pena, Antonio Gonzalez ;
Goodrich, Julia K. ;
Gordon, Jeffrey I. ;
Huttley, Gavin A. ;
Kelley, Scott T. ;
Knights, Dan ;
Koenig, Jeremy E. ;
Ley, Ruth E. ;
Lozupone, Catherine A. ;
McDonald, Daniel ;
Muegge, Brian D. ;
Pirrung, Meg ;
Reeder, Jens ;
Sevinsky, Joel R. ;
Tumbaugh, Peter J. ;
Walters, William A. ;
Widmann, Jeremy ;
Yatsunenko, Tanya ;
Zaneveld, Jesse ;
Knight, Rob .
NATURE METHODS, 2010, 7 (05) :335-336